The effect of deproteinized bovine bone on osteoblast growth factors and proinflammatory cytokine production

Objective To test the ability of Bio-Oss (R) in inducing growth factors and proinflammatory cytokines that may have a role in inflammation after grafting, bone resorption, remodeling and in the homeostasis of osteoblasts. Material and methods Normal human osteoblasts were seeded in Petri dishes containing granules of Bio-Oss (R), cells were harvested after confluency and RNA was extracted. Reverse transcriptase polymerase chain reaction was performed using specific primers for osteonectin, bone sialoprotein (BSP), bone morphogenetic protein (BMP)-2 and BMP-7, platelet-derived growth factor (PDGF), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and integrin beta 1. Glycerol-3-phosphate dehydrogenase was used as the housekeeping gene and normal human osteoblasts grown on Petri dishes without Bio-Oss (R) granules were used as negative controls. Results Osteoblast grown on Bio-Oss (R) showed a normal RNA expression of osteonectin, integrin beta 1 and PDGF. However, compared with control osteoblasts it showed a reduced expression of BSP, BMP-2 and BMP-7, IL-6 and TNF-alpha. Conclusions Our findings further support the evidence that Bio-Oss (R) is an excellent biomaterial that does not enhance the production of proinflammatory cytokines. To cite this article:Amerio P, Vianale G, Reale M, Muraro R, Tulli A, Piattelli A. The effect of deproteinized bovine bone on osteoblast growth factors and proinflammatory cytokine production.Clin. Oral Impl. Res. 21, 2010; 650-655.doi: 10.1111/j.1600-0501.2009.01881.x.