A Polarity-Sensitive Ratiometric Fluorescence Probe for Monitoring Changes in Lipid Droplets and Nucleus during Ferroptosis

被引：243

作者：

Wang, Kang-Nan ^{[1
,2
]}

Liu, Liu-Yi ^{[1
]}

Mao, Duo ^{[2
]}

Xu, Shidang ^{[2
]}

Tan, Cai-Ping ^{[1
]}

Cao, Qian ^{[1
]}

Mao, Zong-Wan ^{[1
]}

Liu, Bin ^{[2
]}

机构：

[1] Sun Yat Sen Univ, Sch Chem, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Peoples R China

[2] Natl Univ Singapore, Dept Chem & Biomol Engn, 4 Engn Dr 4, Singapore 117585, Singapore

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2021年 / 60卷 / 27期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

ferroptosis; fluorescence lifetime imaging; lipid droplets; polarity; ratiometric fluorescent probes; MITOCHONDRIAL POLARITY; CELL-DEATH; BIOLOGY; BINDING;

D O I：

10.1002/anie.202104163

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ferroptosis regulates cell death through reactive oxygen species (ROS)-associated lipid peroxide accumulation, which is expected to affect the structure and polarity of lipid droplets (LDs), but with no clear evidence. Herein, we report the first example of an LD/nucleus dual-targeted ratiometric fluorescent probe, CQPP, for monitoring polarity changes in the cellular microenvironment. Due to the donor-acceptor structure of CQPP, it offers ratiometric fluorescence emission and fluorescence lifetime signals that reflect polarity variations. Using nucleus imaging as a reference, CQPP was applied to report the increase in LD polarity and the homogenization of polarity between LDs and cytoplasm in the ferroptosis model. This LD/nucleus dual-targeted fluorescent probe shows the great potential of using fluorescence imaging to study ferroptosis and ferroptosis-related diseases.

引用

页码：15095 / 15100

页数：6

共 49 条

[11] Ferroptosis: An Iron-Dependent Form of Nonapoptotic Cell Death
Dixon, Scott J.
Lemberg, Kathryn M.
Lamprecht, Michael R.
Skouta, Rachid
Zaitsev, Eleina M.
Gleason, Caroline E.
Patel, Darpan N.
Bauer, Andras J.
Cantley, Alexandra M.
Yang, Wan Seok
Morrison, Barclay, III
Stockwell, Brent R.
[J]. CELL, 2012, 149 (05) : 1060 - 1072
[12] Ferroptosis as a target for protection against cardiomyopathy
Fang, Xuexian
Wang, Hao
Han, Dan
Xie, Enjun
Yang, Xiang
Wei, Jiayu
Gu, Shanshan
Gao, Feng
Zhu, Nali
Yin, Xiangju
Cheng, Qi
Zhang, Pan
Dai, Wei
Chen, Jinghai
Yang, Fuquan
Yang, Huang-Tian
Linkermann, Andreas
Gu, Wei
Min, Junxia
Wang, Fudi
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (07) : 2672 - 2680
[13] Lipid Droplets Finally Get a Little R-E-S-P-E-C-T
Farese, Robert V., Jr.
Walther, Tobias C.
[J]. CELL, 2009, 139 (05) : 855 - 860
[14] Ferroptosis in p53-dependent oncosuppression and organismal homeostasis
Galluzzi, L.
Bravo-San Pedro, J. M.
Kroemer, G.
[J]. CELL DEATH AND DIFFERENTIATION, 2015, 22 (08) : 1237 - 1238
[15] GOUW TH, 1967, FETTE SEIFEN ANSTR V, V69, P223
[16] Recent advances in dual-emission ratiometric fluorescence probes for chemo/biosensing and bioimaging of biomarkers
Gui, Rijun
Jin, Hui
Bu, Xiangning
Fu, Yongxin
Wang, Zonghua
Liu, Qingyun
[J]. COORDINATION CHEMISTRY REVIEWS, 2019, 383 : 82 - 103
[17] Interface-Targeting Strategy Enables Two-Photon Fluorescent Lipid Droplet Probes for High-Fidelity Imaging of Turbid Tissues and Detecting Fatty Liver
Guo, Lifang
Tian, Minggang
Feng, Ruiqing
Zhang, Ge
Zhang, Ruoyao
Li, Xuechen
Liu, Zhiqiang
He, Xiuquan
Sun, Jing Zhi
Yu, Xiaoqiang
[J]. ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (13) : 10706 - 10717
[18] [I-125/I-127]IodoHoechst 33342: Synthesis, DNA binding, and biodistribution
Harapanhalli, RS
McLaughlin, LW
Howell, RW
Rao, DV
Adelstein, SJ
Kassis, AI
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 1996, 39 (24) : 4804 - 4809
[19] The biochemistry of apoptosis
Hengartner, MO
[J]. NATURE, 2000, 407 (6805) : 770 - 776
[20] Live-cell imaging of lipid droplets using solvatochromic coumarin derivatives
Jana, Palash
Siva, Aravintha
Soppina, Virupakshi
Kanvah, Sriram
[J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2020, 18 (29) : 5608 - 5616

← 1 2 3 4 5 →