A Polarity-Sensitive Ratiometric Fluorescence Probe for Monitoring Changes in Lipid Droplets and Nucleus during Ferroptosis

被引：243

作者：

Wang, Kang-Nan ^{[1
,2
]}

Liu, Liu-Yi ^{[1
]}

Mao, Duo ^{[2
]}

Xu, Shidang ^{[2
]}

Tan, Cai-Ping ^{[1
]}

Cao, Qian ^{[1
]}

Mao, Zong-Wan ^{[1
]}

Liu, Bin ^{[2
]}

机构：

[1] Sun Yat Sen Univ, Sch Chem, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Peoples R China

[2] Natl Univ Singapore, Dept Chem & Biomol Engn, 4 Engn Dr 4, Singapore 117585, Singapore

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2021年 / 60卷 / 27期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

ferroptosis; fluorescence lifetime imaging; lipid droplets; polarity; ratiometric fluorescent probes; MITOCHONDRIAL POLARITY; CELL-DEATH; BIOLOGY; BINDING;

D O I：

10.1002/anie.202104163

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ferroptosis regulates cell death through reactive oxygen species (ROS)-associated lipid peroxide accumulation, which is expected to affect the structure and polarity of lipid droplets (LDs), but with no clear evidence. Herein, we report the first example of an LD/nucleus dual-targeted ratiometric fluorescent probe, CQPP, for monitoring polarity changes in the cellular microenvironment. Due to the donor-acceptor structure of CQPP, it offers ratiometric fluorescence emission and fluorescence lifetime signals that reflect polarity variations. Using nucleus imaging as a reference, CQPP was applied to report the increase in LD polarity and the homogenization of polarity between LDs and cytoplasm in the ferroptosis model. This LD/nucleus dual-targeted fluorescent probe shows the great potential of using fluorescence imaging to study ferroptosis and ferroptosis-related diseases.

引用

页码：15095 / 15100

页数：6

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