Panky, a novel photoreceptor-specific ankyrin repeat protein, is a transcriptional cofactor that suppresses CRX-regulated photoreceptor genes

被引：16
作者：
Sanuki, Rikako [1 ,2 ]
Omori, Yoshihiro [1 ]
Koike, Chieko [1 ,3 ]
Sato, Shigeru [1 ]
Furukawa, Takahisa [1 ]
机构：
[1] Osaka Biosci Inst, Dept Dev Biol, Osaka 5650874, Japan
[2] Kyoto Univ, Grad Sch Med, Dept Mol Dev, Sakyo Ku, Kyoto 6068507, Japan
[3] JST, PRESTO, Kawaguchi, Saitama 3320012, Japan
来源：
FEBS LETTERS | 2010年 / 584卷 / 04期
关键词：
Retina; Repressor; Photoreceptor cell; Ankyrin repeat; Transcriptional cofactor; HOMEOBOX GENE; CELL FATE; EXPRESSION; LOCALIZATION; DEGENERATION; HOMEODOMAIN; ATAXIN-7; NETWORK; NRL;
D O I：
10.1016/j.febslet.2009.12.030
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Neuronal gene transcription is regulated by both transcriptional activators and repressors. While the roles of transactivators in retinal photoreceptor development have been well characterized, the roles of repressors have been poorly understood. We isolated Panky/Ankrd33, a gene encoding an ankyrin repeat-containing protein. Panky-A was specifically expressed in retinal photoreceptors and the pineal gland, and its expression was directly up-regulated by the CRX transcription factor. Subcellular localization of PANKY-A was observed in the nucleus and cytoplasm. Additionally, transactivation analysis suggested that PANKY-A is a transcriptional cofactor that suppresses CRX-activated photoreceptor genes. Furthermore, we found by an electrophoretic mobility shift assay that PANKY inhibited the DNA-binding activity of CRX. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：753 / 758
页数：6
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