The indirect UV detection in the analysis of ursodeoxycholic acid and related compounds by HPCE

被引：15

作者：

Quaglia, MG ^{[1
]}

Farina, A ^{[1
]}

Bossu, E ^{[1
]}

DellAquila, C ^{[1
]}

Doldo, A ^{[1
]}

机构：

[1] IST SUPER SANITA,LAB CHIM FARMACO,I-00161 ROME,ITALY

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1997年 / 16卷 / 02期

关键词：

capillary zone electrophoresis; indirect UV detection; ursodeoxycholic acid; impurities determination;

D O I：

10.1016/S0731-7085(97)00034-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance capillary zone electrophoretic (HPCE) assay has been developed for the determination of ursodeoxycholic acid (UDCA) and its usual impurities. Considering the low molecular absorptivity of UDCA and its related compounds indirect UV detection was used. The electrophoretic capillary was filled with a background electrolyte (BGE) containing an UV absorbing ion: benzoic acid (BA) or 5,5-diethylbarbituric acid (DBA). To enhance the selectivity of the assay diimethyl-beta-cyclodextrines (D-beta-CDs) or trimethyl-beta-cyclodextrines (T-beta-CDs) have been added to the running buffer together with methylcellulose or urea. All considered impurities were well resolved with two buffers studied, with the exception of methylursodehoxycholate, a neutral compound. (C) 1997 Elsevier Science B.V.

引用

页码：281 / 285

页数：5

共 12 条

[1] CHOLANIC ACIDS DETERMINED IN COMMERCIAL DRUGS BY MEANS OF A NEW ISFET DEVICE [J].

CAMPANELLA, L ;

BATTILOTTI, M ;

BORRACCINO, A ;

COLAPICCHIONI, C ;

SAMMARTINO, MP ;

TOMASSETTI, M .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1993, 11 (11-12) :1207-1214

[2] INDIRECT PHOTOMETRIC DETECTION IN CAPILLARY ZONE ELECTROPHORESIS [J].

FORET, F ;

FANALI, S ;

OSSICINI, L ;

BOCEK, P .

JOURNAL OF CHROMATOGRAPHY, 1989, 470 (01) :299-308

[3]

FROMM H, 1983, GASTROENTEROLOGY, V85, P1257

[4]

GADACZ TR, 1976, GASTROENTEROLOGY, V70, P1125

[5]

MIYAJI K, 1976, JPN J CLIN EXP MED, V53, P1395

[6]

NARDI A, 1992, B CHIM FARM, V131, P253

[7]

POUPON R, 1987, LANCET, V1, P834

[8] HPLC STUDY OF THE IMPURITIES PRESENT IN DIFFERENT URSODEOXYCHOLIC ACID PREPARATIONS - COMPARATIVE-EVALUATION OF 4 DETECTORS [J].

RODA, A ;

GATTI, R ;

CAVRINI, V ;

CERRE, C ;

SIMONI, P .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1993, 11 (08) :751-760

[9] URSODEOXYCHOLIC ACID VS CHENODEOXYCHOLIC ACID AS CHOLESTEROL GALLSTONE-DISSOLVING AGENTS - A COMPARATIVE RANDOMIZED STUDY [J].

RODA, E ;

BAZZOLI, F ;

LABATE, AMM ;

MAZZELLA, G ;

RODA, A ;

SAMA, C ;

FESTI, D ;

ALDINI, R ;

TARONI, F ;

BARBARA, L .

HEPATOLOGY, 1982, 2 (06) :804-810

[10] INFLUENCE OF HYDROXYLATION AND CONJUGATION OF BILE-SALTS ON THEIR MEMBRANE-DAMAGING PROPERTIES - STUDIES ON ISOLATED HEPATOCYTES AND LIPID-MEMBRANE VESICLES [J].

SCHOLMERICH, J ;

BECHER, MS ;

SCHMIDT, K ;

SCHUBERT, R ;

KREMER, B ;

FELDHAUS, S ;

GEROK, W .

HEPATOLOGY, 1984, 4 (04) :661-666

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