SlPHL1, a MYB-CC transcription factor identified from tomato, positively regulates the phosphate starvation response

被引：21

作者：

Zhang, Yongqiang ^{[1
,2
]}

Wang, Yi ^{[1
]}

Wang, Enhui ^{[1
]}

Wu, Xueqian ^{[1
]}

Zheng, Qinghua ^{[1
]}

Han, Yizhen ^{[1
]}

Lin, Weiwei ^{[1
,2
]}

Liu, Zhongjuan ^{[1
,2
]}

Lin, Wenxiong ^{[1
,2
,3
]}

机构：

[1] Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Fuzhou 350002, Peoples R China

[2] Fujian Prov Univ, Key Lab Crop Ecol & Mol Physiol, Fujian Agr & Forestry Univ, Fuzhou, Peoples R China

[3] Fujian Agr & Forestry Univ, Key Lab Minist Educ Genet Breeding & Multiple Uti, Fuzhou, Peoples R China

来源：

PHYSIOLOGIA PLANTARUM | 2021年 / 173卷 / 03期

基金：

中国国家自然科学基金;

关键词：

PURPLE ACID-PHOSPHATASES; MOLECULAR-MECHANISMS; ARABIDOPSIS; ATPAP10; SYSTEM; RICE; ADAPTATION; METABOLISM; SIGNALS; PROTEIN;

D O I：

10.1111/ppl.13503

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Inorganic phosphate (Pi) deficiency is a major limiting factor for plant growth and development. Previous reports have demonstrated that PHOSPHATE STARVATION RESPONSE 1 (PHR1) and OsPHR2 play central roles in Pi-starvation signaling in Arabidopsis and rice, respectively. However, the Pi-starvation signaling network in tomato (Solanum lycopersicum) is still not fully understood. In this work, SlPHL1, a homolog of AtPHR1 and OsPHR2, was identified from tomato. It was found that SlPHL1 contains the MYB and coiled-coil (CC) domains, localizes in the nucleus, and has transcriptional activity, indicating that it is a typical MYB-CC transcription factor (TF). Overexpression of SlPHL1 enhanced Pi-starvation responses both in Arabidopsis Col-0 and in tomato Micro-Tom, including elevated root hair growth, promoted APase activity, favored Pi uptake, and increased transcription of Pi starvation-inducing (PSI) genes. Besides, overexpressing SlPHL1 was able to compensate for the Pi-starvation response weakened by the AtPHR1 mutation. Notably, electrophoretic mobility shift assay (EMSA) showed that SlPHL1 could bind to the PHR1-binding sequence (P1BS, GNATATNC)-containing DNA fragments. Furthermore, SlPHL1 specifically interacted with the promoters of the tomato PSI genes SlPht1;2 and SlPht1;8 through the P1BS cis-elements. Taken these results together, SlPHL1 is a newly identified MYB-CC TF from tomato, which participates in Pi-starvation signaling by directly upregulating the PSI genes. These findings might contribute to the understanding of the Pi-starvation signaling in tomato.

引用

页码：1063 / 1077

页数：15