Cells of Extraembryonic Mesodermal Origin Confer Human Dystrophin in the Mdx Model of Duchenne Muscular Dystrophy

被引：26

作者：

Kawamichi, Yayoi ^{[2
,3
]}

Cui, Chang-Hao

Toyoda, Masashi

Makino, Hatsune

Horie, Akane

Takahashi, Yoriko

Matsumoto, Kenji ^{[4
]}

Saito, Hirohisa ^{[4
]}

Ohta, Hiroaki ^{[2
]}

Saito, Kayoko ^{[3
]}

Umezawa, Akihiro ^{[1
]}

机构：

[1] Natl Inst Child Hlth & Dev, Dept Reprod Biol, Setagaya Ku, Tokyo 1578535, Japan

[2] Tokyo Womens Med Univ, Dept Obstet & Gynecol, Tokyo, Japan

[3] Tokyo Womens Med Univ, Inst Med Genet, Tokyo, Japan

[4] Natl Inst Child Hlth & Dev, Dept Allergy & Immunol, Tokyo 1578535, Japan

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2010年 / 223卷 / 03期

关键词：

MESENCHYMAL STEM-CELLS; MARROW STROMAL CELLS; BLOOD-DERIVED CELLS; LIFE-SPAN; EXPRESSION; SKELETAL; THERAPY; MUSCLES; GENE;

D O I：

10.1002/jcp.22076

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Duchenne muscular dystrophy is an X-linked recessive genetic disease characterized by severe skeletal muscular degeneration. The placenta is considered to be a promising candidate cell source for cellular therapeutics because it contains a large number of cells and heterogenous cell populations with myogenic potentials. We analyzed the myogenic potential of cells obtained from six parts of the placenta, that is, umbilical cord, amniotic epithelium, amniotic mesoderm, chorionic plate, villous chorion, and decidua basalis. In vitro cells derived from amniotic mesoderm, chorionic plate, and villous chorion efficiently transdifferentiate into myotubes. In addition, in vivo implantation of placenta-derived cells into dystrophic muscles of immunodeficient mdx mice restored sarcolemmal expression of human dystrophin. Differential contribution to myogenesis in this study may be attributed to placental portion-dependent default cell state. Molecular taxonomic characterization of placenta-derived maternal and fetal cells in vitro will help determine the feasibility of cell-based therapy. J. Cell. Physiol. 223: 695-702, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：695 / 702

页数：8

共 24 条

[1] Aminoglycoside antibiotics restore dystrophin function to skeletal muscles of mdx mice [J].