Effects of Gamma Radiation on Bone-Marrow Stromal Cells

被引:10
作者
Zhang, Zengli [1 ,3 ]
Zhang, Hongtao [2 ]
Liu, Fenju [1 ]
Qiu, Mingcai [3 ]
Tong, Jian [1 ]
机构
[1] Soochow Univ, Dept Toxicol, Sch Radiat Med & Publ Hlth, Suzhou 215123, Jiangsu, Peoples R China
[2] Soochow Univ, Dept Orthoped, Affiliated Hosp 1, Suzhou 215123, Jiangsu, Peoples R China
[3] TianJing Med Univ, Dept Endocrinol, Gen Hosp, Tianjin, Peoples R China
来源
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES | 2010年 / 73卷 / 07期
基金
中国博士后科学基金;
关键词
MESENCHYMAL STEM-CELLS; IONIZING-RADIATION; PROGENITOR CELLS; MICROENVIRONMENT; SUBPOPULATIONS; PROLIFERATION; IRRADIATION; CULTURES; TISSUES; GROWTH;
D O I
10.1080/15287390903523477
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The effects of in vitro irradiation on proliferation and hematopoietic supportive functions of stromal cells were studied. To assess the effects of radiation on stromal cells proliferation, marrow cells were exposed to a single dose of gamma radiation. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that stromal cell proliferation was significantly suppressed after radiation in a dose-dependent manner. Stromal layers obtained from irradiated marrow cells failed to establish adherent layers after 6, 8, or 10 Gy of radiation. To assess the functions of stromal cells that survived radiation, stromal layers derived from irradiated marrow cells were cocultured with freshly isolated autologous hematopoietic cells and assayed for their capacity to support prolonged granulocyte-macrophage progenitors (CFU-GM) production. Stromal layers derived from 2-Gy-irradiated marrow cells resulted in similar CFU-GM production as control cells, while stromal layers derived from 4- to 10-Gy-irradiated marrow cells significantly decreased CFU-GM production. To study the influence of radiation on hematopoietic supportive capacity in established stromal layers, stromal layers generated from non-irradiated marrow cells were irradiated and cocultured with freshly isolated autologous hematopoietic cells. Established stromal layers irradiated up to 10 Gy sustained prolonged CFU-GM production, suggesting that hematopoietic stromal supportive functions remained intact at this dose of radiation. In conclusion, our results indicated that proliferation of stromal cells and bone-marrow stromal layer formation from stromal cells are sensitive to radiation in vitro, while established bone-marrow stromal layer originating from stromal cells is relatively resistant to radiation. Data generated may have implications in future bone-marrow transplantation research.
引用
收藏
页码:514 / 519
页数:6
相关论文
共 19 条
[1]  
BOSWELL HS, 1987, EXP HEMATOL, V15, P46
[2]   Osteoblastic cells regulate the haematopoietic stem cell niche [J].
Calvi, LM ;
Adams, GB ;
Weibrecht, KW ;
Weber, JM ;
Olson, DP ;
Knight, MC ;
Martin, RP ;
Schipani, E ;
Divieti, P ;
Bringhurst, FR ;
Milner, LA ;
Kronenberg, HM ;
Scadden, DT .
NATURE, 2003, 425 (6960) :841-846
[3]  
Conget PA, 1999, J CELL PHYSIOL, V181, P67, DOI 10.1002/(SICI)1097-4652(199910)181:1<67::AID-JCP7>3.0.CO
[4]  
2-C
[5]  
Dexter T M, 1984, Prog Clin Biol Res, V148, P13
[6]   Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation [J].
Epperly, Michael W. ;
Epperly, Laura D. ;
Niu, YunYun ;
Wang, Hong ;
Zhang, Xichen ;
Franicola, Darcy ;
Greenberger, Joel S. .
RADIATION RESEARCH, 2007, 168 (05) :560-566
[7]   Hematopoietic cell renewal systems: mechanisms of coping and failing after chronic exposure to ionizing radiation [J].
Fliedner, Theodor M. ;
Graessle, Dieter H. .
RADIATION AND ENVIRONMENTAL BIOPHYSICS, 2008, 47 (01) :63-69
[8]   STROMAL CELLS RESPONSIBLE FOR TRANSFERRING MICROENVIRONMENT OF HEMATOPOIETIC TISSUES - CLONING INVITRO AND RETRANSPLANTATION INVIVO [J].
FRIEDENSTEIN, AJ ;
CHAILAKHYAN, RK ;
LATSINIK, NV ;
PANASYUK, AF ;
KEILISSB.IV .
TRANSPLANTATION, 1974, 17 (04) :331-340
[9]   A Stro-1+ human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system [J].
Goncalves, Raquel ;
da Silva, Claudia Lobato ;
Cabral, Joaquirn M. S. ;
Zanjani, Esmail D. ;
Almeida-Porada, Graca .
EXPERIMENTAL HEMATOLOGY, 2006, 34 (10) :1353-1359
[10]  
Greenberger JS, 1996, LEUKEMIA, V10, P514