Physicochemical properties and activities of comfrey polysaccharides extracted by different techniques

被引：60
作者：
Duan, Mengying [1 ]
Shang, Hongmei [1 ,2 ]
Chen, Shilun [1 ]
Li, Ran [1 ]
Wu, Hongxin [3 ]
机构：
[1] Jilin Agr Univ, Coll Anim Sci & Technol, 2888 Xin Cheng St, Changchun 130118, Jilin, Peoples R China
[2] Jilin Agr Univ, Key Lab Anim Nutr & Feed Sci Jilin Prov, Changchun 130118, Jilin, Peoples R China
[3] CAAS, Grassland Res Inst, Neimenggu 010010, Hohhot, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 115卷
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
Comfrey polysaccharides; Extraction techniques; Physicochemical characteristic; HOHENBUEHELIA-SEROTINA POLYSACCHARIDES; ANTIOXIDANT ACTIVITY; SULFATED MODIFICATION; ENZYMATIC EXTRACTION; BLACK-CURRANT; DRYING METHOD; OPTIMIZATION;
D O I：
10.1016/j.ijbiomac.2018.04.188
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of extraction techniques on the physicochemical properties, antioxidant activity and antihyperglycemic activity of comfrey polysaccharides (CPs) were evaluated. Four techniques were used to extract CPs: hot water extraction (HW), ultrasonic-assisted extraction (UA), enzyme-assisted extraction (EA) and enzyme-ultrasonic-assisted extraction (EUA). Experimental results indicated that CPs extracted by the UA (UA-CPs) and EUA methods (EUA-CPs) had higher extraction yields. The four CPs showed the same monosaccharide composition but a significant difference in monosaccharide content. CPs showed antioxidant activities and antihyperglycemic activities in a concentration-dependent manner. UA-CPs exhibited better antioxidant capacity, which might have been related to its smaller molecular weight and higher uronic acid content. In addition, UA-CPs showed notable a-glucosidase inhibition activity. These results suggested that ultrasonic-assisted extraction technology was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides from comfrey. (C) 2018 Elsevier B.V. All rights reserved.
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页码：876 / 882
页数：7
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