Genetic diversity assessment of Tanzanian sweetpotato genotypes using simple sequence repeat markers

Genetic diversity assessment of 48 Tanzanian sweetpotato genotypes was conducted using nine polymorphic simple sequence repeat markers to determine genetic relationship and select unique parents which could be used for future breeding. Genetic diversity parameters, cluster analysis, and analysis of molecular variance were calculated to determine genetic diversity and relationships. Results showed that the SSR markers used had the mean PIC of 0.78, allelic richness per locus ranged from 4-17 with a mean of 10.0 and the number of effective alleles varied from 2.2-6.1 with a mean value 3.5. The un-weighted pair group method with arithmetic mean allocated the germplasm collection into three major genetic clusters. The greatest genetic distance was identified between the genotypes sourced from Kagera, Temeke, Mkuranga and Kisarawe areas of Tanzania. The study identified genetically unrelated and complementary sweetpotato genotypes such as Ex-Ramadhani, Kibakuli, Mkombozi, Mjomba mkwe, Ex-Halima-3 and Kabuchenji which are recommended for future breeding programmes. (C) 2015 SAAB. Published by Elsevier B.V. All rights reserved.