Chitinase activity of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57)

被引：0

作者：

Hou, WC

Chen, YC

Lin, YH ^{[1
]}

机构：

[1] Acad Sinica, Inst Bot, Taipei 115, Taiwan

[2] Tatung Inst Technol, Dept Bioengn, Taipei 115, Taiwan

来源：

BOTANICAL BULLETIN OF ACADEMIA SINICA | 1998年 / 39卷 / 02期

关键词：

chitinase; defense against pests; Ipomoea batatas;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Chitinase activity was detected in the crude extracts of different pairs of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57) using commercial deacetyl glycol chitin as a substrate. The activity of chitinase in crude extracts, which was expressed as units/g fresh weight, was arranged in decending order of leaves > sprouts > peels of storage roots > peeled storage roots. Under field conditions, the chitinase activity of leaves was higher than vines. The chitinase activity of insect-bitten leaves was higher than that of the controls, and the chitinase activity of vines attached to insect-bitten leaves was also higher than that of vines attached to intact leaves. The molecular weight of main chitinase determined by activity staining was about 16 kDa. The optimum DH was 5.0 and the optimum temperature of chitinase activity was 25 degrees C.

引用

页码：93 / 97

页数：5

共 28 条

[1] PREPARATION AND PURIFICATION OF GLUCANASE AND CHITINASE FROM BEAN LEAVES [J].