Polymorphisms in the ALS gene of weedy rice (Oryza sativa L.) accessions with differential tolerance to imazethapyr

被引:19
作者
Shivrain, Vinod K. [1 ]
Burgos, Nilda R. [1 ]
Sales, Marites A. [1 ]
Kuk, Yong I. [2 ]
机构
[1] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA
[2] Sunchon Natl Univ, Coll Life Sci & Nat Resources, Dept Bioresource Sci, Jeonnam 540742, South Korea
关键词
Acetolactate synthase (EC 4.1.3.18); Genetic polymorphism; Herbicide resistance; Selection pressure; ACETOLACTATE SYNTHASE GENE; AMARANTH AMARANTHUS-PALMERI; RED RICE; INHIBITING HERBICIDES; ARABIDOPSIS-THALIANA; CLEARFIELD(TM) RICE; RESISTANCE; GLYPHOSATE; MUTATIONS; ARKANSAS;
D O I
10.1016/j.cropro.2009.10.002
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Herbicide-resistant Clearfield (TM) rice technology allows the use of ALS inhibitors to control weedy rice. Weedy rice plants have differential tolerance to imazethapyr, perhaps due to ALS polymorphisms. We aimed to assess ALS polymorphisms in weedy rice accessions from Arkansas, USA, with differential tolerance to imazethapyr in seedling growth bioassays. Six base changes were identified in the ALS of 14 weedy rice accessions. Three of these nucleotide changes resulted in amino acid substitutions - Pro(93)Thr, Glu(630)Asp, and Val(669)Met - in four accessions: Ark-4, Ark-9, Poi-1 and Poi-6. The GR(50) values and inhibition of root and shoot growth (%) of these accessions differed. The Glu(630)Asp substitution occurs in the herbicide binding domain B and Vl(669)Met occurs at the C-terminal tail where the co-factor binds. Variability in weedy rice ALS exists, but polymorphism patterns did not relate to tolerance levels. The observed mutations presented the possibility that sustained selection pressure will eventually lead to selection of a herbicide-tolerant individual that will be the progenitor of a resistant population. Concomitantly, pollen-mediated gene flow from Clearfield (TM), rice to weedy rice will lead to the evolution of ALS-resistant weedy rice populations. (C) Published by Elsevier Ltd.
引用
收藏
页码:336 / 341
页数:6
相关论文
共 47 条
  • [1] Effect of flood timing on red rice (Oryza spp.) control with imazethapyr applied at different dry-seeded rice growth stages
    Avila, LA
    Senseman, SA
    McCauley, GN
    Chandler, JM
    O'Barr, JH
    [J]. WEED TECHNOLOGY, 2005, 19 (02) : 476 - 480
  • [2] A NATURALLY-OCCURRING POINT MUTATION CONFERS BROAD RANGE TOLERANCE TO HERBICIDES THAT TARGET ACETOLACTATE SYNTHASE
    BERNASCONI, P
    WOODWORTH, AR
    ROSEN, BA
    SUBRAMANIAN, MV
    SIEHL, DL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (29) : 17381 - 17385
  • [3] Red rice (Oryza sativa) status after 5 years of imidazolinone-resistant rice technology in Arkansas
    Burgos, Nilda R.
    Norsworthy, Jason K.
    Scott, Robert C.
    Smith, Kenneth L.
    [J]. WEED TECHNOLOGY, 2008, 22 (01) : 200 - 208
  • [4] Breeding systems and genome evolution
    Charlesworth, D
    Wright, SI
    [J]. CURRENT OPINION IN GENETICS & DEVELOPMENT, 2001, 11 (06) : 685 - 690
  • [5] A uniform, objective, and adaptive system for expressing rice development
    Counce, PA
    Keisling, TC
    Mitchell, AJ
    [J]. CROP SCIENCE, 2000, 40 (02) : 436 - 443
  • [6] Craigmiles JP., 1978, Texas Agric Exp Stn Bull, V1270, P5
  • [7] Glyphosate-resistant Palmer amaranth (Amaranthus palmeri) confirmed in Georgia
    Culpepper, A. Stanley
    Grey, Timothy L.
    Vencill, William K.
    Kichler, Jeremy M.
    Webster, Theodore M.
    Brown, Steve M.
    York, Alan C.
    Davis, Jerry W.
    Hanna, Wayne W.
    [J]. WEED SCIENCE, 2006, 54 (04) : 620 - 626
  • [8] Devine MD., 1997, Herbicide Activity: Toxicology, Biochemistry and Molecular Biology, P159
  • [9] Doyle JJ, 1987, PHYTOCHEM B, V19, P11, DOI DOI 10.2307/4119796
  • [10] MULTIPLE RESISTANCE TO SULFONYLUREAS AND IMIDAZOLINONES CONFERRED BY AN ACETOHYDROXY ACID SYNTHASE GENE WITH SEPARATE MUTATIONS FOR SELECTIVE RESISTANCE
    HATTORI, J
    RUTLEDGE, R
    LABBE, H
    BROWN, D
    SUNOHARA, G
    MIKI, B
    [J]. MOLECULAR AND GENERAL GENETICS, 1992, 232 (02): : 167 - 173