Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats

被引:6
作者
Barbe, Caroline [1 ]
Salles, Jerome [1 ]
Chambon, Christophe [2 ,3 ]
Giraudet, Christophe [1 ]
Sanchez, Phelipe [1 ]
Patrac, Veronique [1 ]
Denis, Philippe [1 ]
Boirie, Yves [1 ,4 ]
Walrand, Stephane [1 ,4 ]
Gueugneau, Marine [1 ]
机构
[1] Univ Clermont Auvergne, Human Nutr Unit, Auvergne Human Nutr Res Ctr, INRAE, F-63000 Clermont Ferrand, France
[2] Anim Prod Qual Unit QuaPA, INRAE, F-63122 Clermont Ferrand, France
[3] Univ Clermont Auvergne, Metabol & Prote Explorat Facil, INRAE, F-63122 Clermont Ferrand, France
[4] Clermont Ferrand Univ Hosp Ctr, Dept Clin Nutr, F-63000 Clermont Ferrand, France
关键词
skeletal muscle; aging; sarcopenia; undernutrition; proteome; DIETARY RESTRICTION; INSULIN-RESISTANCE; AGED RATS; METABOLISM; STRENGTH; EXPRESSION; PROTEINS; PATHWAY; MODEL; YOUNG;
D O I
10.3390/ijms23094762
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Aging is associated with a progressive loss of skeletal muscle mass and function termed sarcopenia. Various metabolic alterations that occur with aging also increase the risk of undernutrition, which can worsen age-related sarcopenia. However, the impact of undernutrition on aged skeletal muscle remains largely under-researched. To build a deeper understanding of the cellular and molecular mechanisms underlying age-related sarcopenia, we characterized the undernutrition-induced changes in the skeletal muscle proteome in old rats. For this study, 20-month-old male rats were fed 50% or 100% of their spontaneous intake for 12 weeks, and proteomic analysis was performed on both slow- and fast-twitch muscles. Proteomic profiling of undernourished aged skeletal muscle revealed that undernutrition has profound effects on muscle proteome independently of its effect on muscle mass. Undernutrition-induced changes in muscle proteome appear to be muscle-type-specific: slow-twitch muscle showed a broad pattern of differential expression in proteins important for energy metabolism, whereas fast-twitch muscle mainly showed changes in protein turnover between undernourished and control rats. This first proteomic analysis of undernourished aged skeletal muscle provides new molecular-level insight to explain phenotypic changes in undernourished aged muscle. We anticipate this work as a starting point to define new biomarkers associated with undernutrition-induced muscle loss in the elderly.
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页数:19
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