CRISPR/Cas9 Gene-Editing in Cancer Immunotherapy: Promoting the Present Revolution in Cancer Therapy and Exploring More

被引：39

作者：

Ou, Xuejin ^{[1
,2
]}

Ma, Qizhi ^{[2
]}

Yin, Wei ^{[3
]}

Ma, Xuelei ^{[1
]}

He, Zhiyao ^{[1
,4
,5
]}

机构：

[1] Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Dept Biotherapy, Chengdu, Peoples R China

[2] Sichuan Univ, West China Hosp, Dept Thorac Oncol, Chengdu, Peoples R China

[3] Sichuan Univ, West China Sch Med, Chengdu, Peoples R China

[4] Sichuan Univ, West China Hosp, Natl Clin Res Ctr Geriatr, Dept Pharm,State Key Lab Biotherapy, Chengdu, Peoples R China

[5] Sichuan Univ, West China Hosp, Natl Clin Res Ctr Geriatr, Canc Ctr, Chengdu, Peoples R China

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2021年 / 9卷

关键词：

CRISPR; Cas9; immunotherapy; TCR-T; tumor-infiltrating lymphocytes; CAR-T; TUMOR-INFILTRATING LYMPHOCYTES; CHIMERIC-ANTIGEN-RECEPTOR; T-CELLS; METASTATIC MELANOMA; ADOPTIVE TRANSFER; NIVOLUMAB; CTLA-4; PD-1; EXHAUSTION; TARGET;

D O I：

10.3389/fcell.2021.674467

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In recent years, immunotherapy has showed fantastic promise in pioneering and accelerating the field of cancer therapy and embraces unprecedented breakthroughs in clinical practice. The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (CRISPR-Cas9) system, as a versatile gene-editing technology, lays a robust foundation to efficiently innovate cancer research and cancer therapy. Here, we summarize recent approaches based on CRISPR/Cas9 system for construction of chimeric antigen receptor T (CAR-T) cells and T cell receptor T (TCR-T) cells. Besides, we review the applications of CRISPR/Cas9 in inhibiting immune checkpoint signaling pathways and highlight the feasibility of CRISPR/Cas9 based engineering strategies to screen novel cancer immunotherapy targets. Conclusively, we discuss the perspectives, potential challenges and possible solutions in this vivid growing field.

引用

页数：12

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