Element-to-tissue correlation in biological samples determined by three-dimensional X-ray imaging methods

被引：60

作者：

De Samber, Bjoern ^{[1
]}

Silversmit, Geert ^{[1
]}

De Schamphelaere, Karel ^{[2
]}

Evens, Roel ^{[2
]}

Schoonjans, Tom ^{[1
]}

Vekemans, Bart ^{[1
]}

Janssen, Colin ^{[2
]}

Masschaele, Bert ^{[3
]}

Van Hoorebeke, Luc ^{[3
]}

Szaloki, Imre ^{[4
]}

Vanhaecke, Frank ^{[1
]}

Rickers, Karen ^{[5
]}

Falkenberg, Gerald ^{[5
]}

Vincze, Laszlo ^{[1
]}

机构：

[1] Univ Ghent, Dept Analyt Chem, B-9000 Ghent, Belgium

[2] Univ Ghent, Lab Environm Toxicol & Aquat Ecol, B-9000 Ghent, Belgium

[3] Univ Ghent, Dept Subatom & Radiat Phys, Ctr Xray Tomog, B-9000 Ghent, Belgium

[4] Budapest Univ Technol & Econ, Inst Nucl Tech, H-1111 Budapest, Hungary

[5] Hamburger Synchrotronstrahlungslabor DESY, D-22603 Hamburg, Germany

来源：

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY | 2010年 / 25卷 / 04期

关键词：

DAPHNIA-MAGNA; FLUORESCENCE; ZINC; WATERBORNE; TOXICITY; SYNCHROTRON; EXPOSURE; MATTER;

D O I：

10.1039/b918624g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Synchrotron radiation based confocal micro-XRF was employed to unravel the tissue-specific three-dimensional (3D) distribution of metals down to trace concentration levels in a non-destructive manner within the crustacean Daphnia magna, an ecotoxicological model organism. Next to the analytical characterization of the employed confocal micro-XRF set-up, specific areas of metal accumulation in different cross-sections of interest within the organism were investigated. The use of a fast dynamic (continuous) scanning approach delivered 3D information on major, minor and trace element distributions in specific sub-regions within Daphnia magna. Coupling the obtained elemental information with microscopic morphological data obtained by laboratory absorption microtomography, full 3D element-to-tissue correlation could be derived, allowing a more detailed interpretation of the obtained results with respect to metal accumulation within this model organism.

引用

页码：544 / 553

页数：10

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