Detection of Leishmania (Viannia) DNA in blood from patients with American cutaneous leishmaniasis

被引：19
作者：
Venazzi, E. A. S.
Roberto, A. C. B. S.
Barbosa-Tessmann, I. P.
Zanzarini, P. D.
Lonardoni, M. V. C.
Silveira, T. G. V.
机构：
[1] Univ Estadual Maringa, Dept Anal Clin, BR-87020900 Maringa, Parana, Brazil
[2] Univ Estadual Maringa, Dept Bioquim, BR-87020900 Maringa, Parana, Brazil
来源：
EXPERIMENTAL PARASITOLOGY | 2007年 / 115卷 / 04期
关键词：
cutaneous leishmaniasis; Leishmania braziliensis; blood; PCR; POLYMERASE-CHAIN-REACTION; MUCOCUTANEOUS LEISHMANIASIS; DIAGNOSIS; BRAZILIENSIS; EXTRACTION; LESIONS; PCR;
D O I：
10.1016/j.exppara.2006.10.002
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The aim of the present study was to investigate the presence of Leishmania (Viannia) subgenus DNA in peripheral blood from patients with cutaneous lesions due to American cutaneous leishmaniasis. The buffy coats from 68 blood samples were analyzed by polymerase chain reaction using the MP1L/MP3H primers. The parasite DNA was detected in 2 (3.4%) out of 59 patients who had amastigotes present in samples taken from lesions. The presence of Leishmania (Viannia) DNA in the blood of these patients indicates hematogeneous parasite dissemination. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：399 / 402
页数：4
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