Lymphocytes upregulate signal sequence-encoding proopiomelanocortin mRNA and beta-endorphin during painful inflammation in vivo

被引：52
作者：
Sitte, Nicolle
Busch, Melanie
Mousa, Shaaban A.
Labuz, Dominika
Rittner, Heike
Gore, Carmen
Krause, Hans
Stein, Christoph
Schaefer, Michael
机构：
[1] Charite Univ Med Berlin, Dept Anesthesiol & Crit Care Med, D-12200 Berlin, Germany
[2] Charite Univ Med Berlin, Dept Urol, D-12200 Berlin, Germany
来源：
JOURNAL OF NEUROIMMUNOLOGY | 2007年 / 183卷 / 1-2期
关键词：
opioid peptides; pain; immune system; Complete Freund's Adjuvant; neuro-immune interaction;
D O I：
10.1016/j.jneuroim.2006.11.033
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Proopiomelanocortin (POMC)-derived beta-endorphin(1-31) (END) released from immune cells inhibits inflammatory pain. We examined the expression of END and POMC mRNA encoding the signal sequence required for entry of the nascent polypeptide into the regulated secretory pathway in lymphocytes of rats with inflamed hindpaws. Within 12 h of inflammation, END increased in popliteal lymph nodes and at 96 It the intraplantar neutralization of END exacerbated pain. Lymphocytes expressed POMC, END, and full-length POMC mRNA. Semi-nested PCR revealed 8-fold increased exon 2-3 spanning POMC mRNA. Thus, painful inflammation enhances signal sequence-encoding lymphocytic POMC mRNA needed for regulated secretion of functionally active END. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：133 / 145
页数：13
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