Conjugated linoleic acid suppresses colon carcinogenesis in azoxymethane-pretreated rats with long-term feeding of diet containing beef tallow

被引：30

作者：

Shiraishi, Ryosuke ^{[1
]}

Iwakiri, Ryuichi ^{[1
]}

Fujise, Takehiro ^{[1
]}

Kuroki, Tsukasa ^{[1
]}

Kakimoto, Takashi ^{[1
]}

Takashima, Tooru ^{[1
]}

Sakata, Yasuhisa ^{[1
]}

Tsunada, Seiji ^{[1
]}

Nakashima, Yutaka ^{[2
]}

Yanagita, Teruyoshi ^{[3
]}

Fujimoto, Kazuma ^{[1
]}

机构：

[1] Saga Med Sch, Dept Internal Med & Gastrointestinal Endoscopy, Saga 8498501, Japan

[2] Fujioka Hosp, Saga 8400201, Japan

[3] Saga Univ, Dept Appl Biochem & Food Sci, Saga 8408502, Japan

来源：

JOURNAL OF GASTROENTEROLOGY | 2010年 / 45卷 / 06期

基金：

日本学术振兴会;

关键词：

Aberrant crypt foci; Cell proliferation; Apoptosis; Beta catenin; Cyclooxygenase; 2; ABERRANT CRYPT FOCI; BIOLOGICALLY-ACTIVE ISOMERS; CANCER CELL-PROLIFERATION; KAPPA-B ACTIVATION; BODY-FAT MASS; F344; RATS; TRANS-11; INHIBITION; CIS-12; RISK;

D O I：

10.1007/s00535-010-0206-8

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

We have indicated previously that long-term feeding of beef tallow increases colorectal cancer in rats. In this study, we investigated the effects of conjugated linoleic acid (CLA) on colon carcinogenesis in rats under long-term feeding of beef tallow diets, pretreated with azoxymethane (AOM). Six-week-old male Sprague-Dawley rats were fed with 10% beef tallow diet only, 10% beef tallow with 1% CLA in triglyceride form (CLA-TG), or 10% beef tallow with 1% CLA in free fatty acid form (CLA-FFA). Colon carcinogenesis was induced by two intraperitoneal injections of AOM. Aberrant crypt foci (ACFs) were examined at 12 weeks. Cancer, cell proliferation, apoptosis, Wnt signaling, and the arachidonic acid cascade were examined at 44 weeks. At 12 weeks, CLA-TG and CLA-FFA attenuated the increase in ACFs induced by 10% beef tallow and AOM pretreatment. At 44 weeks, both forms of CLA attenuated multiple colon cancers, and CLA-FFA reduced the incidence of colon cancer to 50% of that seen with CLA-TG. CLA-TG and CLA-FFA decreased the number of 5-bromo-2'-deoxyuridine-positive cells in AOM-pretreated rats fed with 10% beef tallow. CLA-FFA increased the number of apoptotic cells and the activity of caspase-3 in the colon mucosa, and CLA-TG enhanced the activity of caspase-3. Both forms of CLA suppressed Wnt signaling and the arachidonic acid cascade in rats treated with beef tallow and AOM. These results suggested that CLA-TG and CLA-FFA suppressed colon carcinogenesis in rats with long-term feeding of a 10% beef tallow diet, through several mechanisms. The results of the present study with rats might be applicable to humans.

引用

页码：625 / 635

页数：11

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