Isotopic heterogeneity and cycling of organic nitrogen in the oligotrophic ocean

We measured the nitrogen (N) isotopic composition (delta N-15) of a large set (n = 38) of high-molecular-weight (HMW) dissolved organic nitrogen (DON) samples isolated from the tropical and subtropical North Atlantic and Pacific Oceans. The delta N-15 signature of surface HMW DON is relatively invariable in both oligotrophic basins (4.1 +/- 0.6 in the Atlantic; 5.4 +/- 0.8 in the Pacific) and shows little correlation with sources or concentrations of N supporting new production in the euphotic zone. While large variations in delta N-15 of bulk HMW DON are not apparent, delta N-15 of proteins isolated from sites with relatively high rates of N-2 fixation (> 80 mu mol N m(-2) d(-1)) were consistently depleted in N-15 relative to bulk HMW DON and to proteins isolated from sites where N-2 fixation does not routinely occur. This small component of HMW DON appears to be cycling more rapidly than bulk HMW DON and may be indicative of fresh DON contributed by organisms in the surface ocean. Furthermore, delta N-15 of DNA extracted from the bacterial size fraction (0.2 - 0.5 mu m) revealed that free-living bacteria may be an important sink for isotopically depleted N produced during N-2 fixation. We suggest that there exists a tight coupling between the production and uptake of DON contributed by diazotrophs (N-2 fixers) in regions where N-2 fixation provides a major input of new nitrogen.