The effect of G-CSF and AMD3100 on mice treated with streptozotocin: Expansion of alpha-cells and partial islet protection

被引:5
作者
Gomez, Yonathan [1 ,2 ]
Diaz-Solano, Dylana [1 ]
Gledhill, Teresa [3 ]
Wittig, Olga [1 ]
Cardier, Jose [1 ]
机构
[1] IVIC, Ctr Med Expt, Unidad Terapia Celular, Lab Patol Celular & Mol, Apartado 21827, Caracas 1020A, Venezuela
[2] Univ Cent Venezuela, Fac Ciencias, Caracas 1080, Venezuela
[3] Hosp Jose Maria Vargas, Serv Anatomopatol, Caracas 1080, Venezuela
关键词
G-CSF; AMD3100; Streptozotocin; Alpha-cells; Beta-cells; Diabetes; HEMATOPOIETIC STEM-CELLS; LIVER-INJURY; MOBILIZATION; REPAIR; PROGENITORS; MECHANISMS;
D O I
10.1016/j.cyto.2017.04.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Administration of streptozotocin (STZ) is one of the most used experimental models of diabetes (STZ-DT). STZ induces beta-cell damage in pancreatic islets. It is known that hematopoietic stem progenitor cells (HSPCs) are mobilized from bone marrow to damaged tissues. In this work, we evaluated the effects of the hematopoietic mobilizers G-CSF (250 pg/kg; for five consecutive days) and AMD3100 (5 mg/kg; single s.c injection) in mice treated with STZ (175 mg/kg). Mice injected with STZ showed a significant reduction in the number and area of islets and in the number of beta- and alpha-cells. Concurrently, they had hyperglycemia (blood glucose over 300 mg/dl) associated with very low levels of insulin in plasma. The number and area of islets from STZ-DT mice treated with G-CSF and/or AMD3100 were similar to the controls. However, these mice had neither a reduction of hyperglycemia nor an improvement in the insulin levels. Analysis of islet cellularity showed a large reduction in beta-cells with a significant expansion of alpha-cells. These results indicate that G-CSF and AMD3100 induce partial protection of islet tissues and expansion of alpha-cells in mice treated with STZ but do not protect beta cells from the damage induced by this compound.
引用
收藏
页码:123 / 131
页数:9
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