Labeling and Imaging of Stem Cells - Promises and Concerns

被引：8
作者：
Schaefer, Richard [1 ]
机构：
[1] Univ Tubingen Hosp, Inst Clin & Expt Transfus Med, Tubingen, Germany
来源：
TRANSFUSION MEDICINE AND HEMOTHERAPY | 2010年 / 37卷 / 02期
关键词：
Labeling; Imaging; Stem cells; SPIO; MRI; MAGNETIC-RESONANCE TRACKING; SUPERPARAMAGNETIC IRON-OXIDES; IN-VIVO TRACKING; PROGENITOR CELLS; CONTRAST AGENT; TRANSFERRIN RECEPTOR; MELANOMA PATIENTS; DENDRITIC CELLS; SINGLE CELLS; L-LYSINE;
D O I：
10.1159/000287271
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
For experimental and clinical applications of stem cells cell labeling and tracking is interesting to evaluate cell distribution and homing. Tracking of cells after transplantation can be performed for monitoring the delivery and faith of the graft. Furthermore, imaging techniques are part of the evaluation of the functional effects of cellular therapy in the organism of the host. Therefore, systematic investigations on the development and clinical evaluation of imaging techniques and their possible biological impact on stem cells is an emerging topic of today's applied stem cell research. This article refers to different labeling techniques of stem cells highlighting their promises for clinical use and discussing their possible risks.
引用
收藏
页码：85 / 89
页数：5
相关论文
共 62 条
  • [41] Expression of transferrin receptor and ferritin following ferumoxides-protamine sulfate labeling of cells: implications for cellular magnetic resonance imaging
    Pawelczyk, Edyta
    Arbab, Ali S.
    Pandit, Sunil
    Hu, Elbert
    Frank, Joseph A.
    [J]. NMR IN BIOMEDICINE, 2006, 19 (05) : 581 - 592
  • [42] Molecular MRI of hematopoietic stem-progenitor cells:: in vivo monitoring of gene therapy and atherosclerosis
    Qiu, Bensheng
    Yang, Xiaoming
    [J]. NATURE CLINICAL PRACTICE CARDIOVASCULAR MEDICINE, 2008, 5 (07): : 396 - 404
  • [43] RECEPTOR IMAGING - APPLICATION TO MR IMAGING OF LIVER-CANCER
    REIMER, P
    WEISSLEDER, R
    LEE, AS
    WITTENBERG, J
    BRADY, TJ
    [J]. RADIOLOGY, 1990, 177 (03) : 729 - 734
  • [44] Aptamer-based isolation and subsequent imaging of mesenchymal stem cells in ischemic myocard by magnetic resonance imaging
    Schaefer, R.
    Wiskirchen, J.
    Guo, K.
    Neumann, B.
    Kehlbach, R.
    Pintaske, J.
    Voth, V.
    Walker, T.
    Scheule, A. M.
    Greiner, T. O.
    Hermanutz-Klein, U.
    Claussen, C. D.
    Northoff, H.
    Ziemer, G.
    Wendel, H. P.
    [J]. ROFO-FORTSCHRITTE AUF DEM GEBIET DER RONTGENSTRAHLEN UND DER BILDGEBENDEN VERFAHREN, 2007, 179 (10): : 1009 - 1015
  • [45] Basic research and clinical applications of non-hematopoietic stem cells, 4-5 April 2008, Tubingen, Germany
    Schaefer, R.
    Dominici, M.
    Mueller, I.
    Horwitz, E.
    Asahara, T.
    Bulte, J. W. M.
    Bieback, K.
    Le Blanc, K.
    Buehring, H. J.
    Capogrossi, M. C.
    Dazzi, F.
    Gorodetsky, R.
    Henschler, R.
    Handgretinger, R.
    Kajstura, J.
    Kluger, P. J.
    Lange, C.
    von Luettichau, I.
    Mertsching, H.
    Schrezenmeier, H.
    Sievert, K. D.
    Strunk, D.
    Verfaillie, C.
    Northoff, H.
    [J]. CYTOTHERAPY, 2009, 11 (02) : 245 - 255
  • [46] Transferrin receptor upregulation:: In vitro labeling of rat mesenchymal stem cells with superparamagnetic iron oxide
    Schaefer, Richard
    Kehlbach, Rainer
    Wiskirchen, Jakub
    Bantleon, Ruediger
    Pintaske, Joerg
    Brehm, Bernhard R.
    Gerber, Annika
    Wolburg, Hartwig
    Claussen, Claus D.
    Northoff, Hinnak
    [J]. RADIOLOGY, 2007, 244 (02) : 514 - 523
  • [47] Labeling of human mesenchymal stromal cells with superparamagnetic iron oxide leads to a decrease in migration capacity and colony formation ability
    Schaefer, Richard
    Kehlbach, Rainer
    Mueller, Michaela
    Bantleon, Ruediger
    Kluba, Torsten
    Ayturan, Miriam
    Siegel, Georg
    Wolburg, Hartwig
    Northoff, Hinnak
    Dietz, Klaus
    Claussen, Claus D.
    Wiskirchen, Jakub
    [J]. CYTOTHERAPY, 2009, 11 (01) : 68 - 78
  • [48] The Use of Clinically Approved Small Particles of Iron Oxide (SPIO) for Labeling of Mesenchymal Stem Cells Aggravates Clinical Symptoms in Experimental Autoimmune Encephalomyelitis and Influences Their In Vivo Distribution
    Schaefer, Richard
    Ayturan, Miriam
    Bantleon, Ruediger
    Kehlbach, Rainer
    Siegel, Georg
    Pintaske, Joerg
    Conrad, Sabine
    Wolburg, Hartwig
    Northoff, Hinnak
    Wiskirchen, Jakub
    Weissert, Robert
    [J]. CELL TRANSPLANTATION, 2008, 17 (08) : 923 - 941
  • [49] Intracellular magnetic labeling of lymphocytes for in vivo trafficking studies
    Schoepf, U
    Marecos, EM
    Melder, RJ
    Jain, RK
    Weissleder, R
    [J]. BIOTECHNIQUES, 1998, 24 (04) : 642 - +
  • [50] In vivo detection of single cells by MRI
    Shapiro, EM
    Sharer, K
    Skrtic, S
    Koretsky, AP
    [J]. MAGNETIC RESONANCE IN MEDICINE, 2006, 55 (02) : 242 - 249
1234567