LncRNA TNRC6C-AS1 regulates UNC5B in thyroid cancer to influence cell proliferation, migration, and invasion as a competing endogenous RNA of miR-129-5p

被引：37
作者：
Hou, Sen [1 ]
Lin, Qiuyu [1 ]
Guan, Feng [1 ]
Lin, Chenghe [1 ]
机构：
[1] Jilin Univ, Hosp 1, Dept Nucl Med, 71 Xinmin St, Changchun 130021, Jilin, Peoples R China
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2018年 / 119卷 / 10期
关键词：
miR-129-5p; thyroid cancer; TNRC6C-AS1; UNC5B; LONG NONCODING RNA; MALAT1; PROMOTES; CARCINOMA; EXPRESSION; TARGET; GROWTH;
D O I：
10.1002/jcb.26868
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To investigate the biological functions and regulatory mechanism of lncRNA TNRC6C-AS1 in thyroid cancer (TC). TNRC6C-AS1, miR-129-5p, and UNC5B expression levels were investigated by qRT-PCR and Western blot. CCK-8 assay was conducted to determine cell proliferation, while transwell assay was for inspection of cell migration and invasion. Through bioinformatic analysis, the interactions among TNRC6C-AS1, miR-129-5p, and UNC5B were predicted. Dual luciferase reporter gene assay and RNA pull-down assay confirmed the predicted target relationships. Tumor xenograft assay was applied to inspect the effect of TNRC6C-AS1 downregulation on TC development in vivo. TNRC6C-AS1 and UNC5B were overexpressed, while miR-129-5p was underexpressed in TC tissues and cells. TNRC6C-AS1/UNC5B downregulation and miR-129-5p overexpression could suppress proliferation, migration, and invasion of TC cells as well as inhibit tumorigenesis in vivo. MiR-129-5p targeted TNRC6C-AS1 and UNC5B in TC cells; and UNC5B expression was downregulated by knocking down TNRC6C-AS1, which competitively bound with miR-129-5p. Downregulation of TNRC6C-AS1 restrained TC development by knocking down UNC5B through upregulating the expression of miR-129-5p.
引用
收藏
页码：8304 / 8316
页数：13
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