The formation of the polyploid hybrids from different subfamily fish crossings and its evolutionary significance

被引：109

作者：

Liu, Shaojun ^{[1
]}

Qin, Qinbo ^{[1
]}

Xiao, Jun ^{[1
]}

Lu, Wenting ^{[1
]}

Shen, Jiamin ^{[1
]}

Li, Wei ^{[1
]}

Liu, Jifang ^{[1
]}

Duan, Wei ^{[1
]}

Zhang, Chun ^{[1
]}

Tao, Min ^{[1
]}

Zhao, Rurong ^{[1
]}

Yan, Jinpeng ^{[1
]}

Liu, Yun ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Life Sci, Educ Minist China, Key Lab Protein Chem & Fish Dev Biol, Changsha 410081, Peoples R China

来源：

GENETICS | 2007年 / 176卷 / 02期

关键词：

D O I：

10.1534/genetics.107.071373

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

This study provides genetic evidences at the chromosome, DNA content, DNA fragment and sequence, and morphological levels to support the successful establishment of the polyploid hybrids of red crucian carp X blunt snout bream, which belonged to a different subfamily of fish (Cyprininae subfamily and Cultrinae subfamily) in the catalog. We successfully obtained the sterile triploid hybrids and bisexual fertile tetraploid hybrids of red crucian carp (RCC) (female) X blunt snout bream (BSB) (male) as well as their pentaploid hybrids. The triploid hybrids possessed 124 chromosomes with two sets from RCC and one set from BSB; the tetraploid hybrids had 148 chromosomes with two sets from RCC and two sets from BSB. The females of tetraploid hybrids produced unreduced tetraploid eggs that were fertilized with the haploid sperm of BSB to generate pentaploid hybrids with 172 chromosomes with three sets from BSB and two sets from RCC. The ploidy levels of triploid, tetraploid, and pentaploid hybrids were confirmed by counting chromosomal number, forming chromosomal karyotype, and measuring DNA content and erythrocyte nuclear volume. The similar and different DNA fragments were PCR amplified and sequenced in triploid, tetraploid hybrids, and their parents, indicating their molecular genetic relationship and genetic markers. In addition, this study also presents results about the phenotypes and feeding habits of polyploid hybrids and discusses the formation mechanism of the polyploid hybrids. It is the first report on the formation of the triploid, tetraploid, and pentaploid hybrids by crossing parents, with a different chromosome number in vertebrates. The formation of the polyploid hybrids is potentially interesting in both evolution and fish genetic breeding.

引用

页码：1023 / 1034

页数：12

共 13 条

[1]

Alves MJ, 1999, GENETICS, V151, P277

[2]

[Anonymous], 1989, Molecular Cloning

[3] CHROMOSOMAL AND ELECTROPHORETIC ANALYSES OF HYBRIDS BETWEEN GRASS CARP AND BIGHEAD CARP (PISCES, CYPRINIDAE) [J].