Investigation of Linezolid Resistance in Staphylococci and Enterococci

被引:24
作者
Doern, Christopher D. [1 ]
Park, Jason Y. [2 ,3 ,4 ]
Gallegos, Michael [4 ,8 ]
Alspaugh, Debbie [5 ]
Burnham, Carey-Ann D. [6 ,7 ]
机构
[1] Virginia Commonwealth Univ, Med Ctr, Dept Pathol, Richmond, VA USA
[2] Univ Texas SW Med Ctr Dallas, Dept Pathol, Dallas, TX 75390 USA
[3] Univ Texas SW Med Ctr Dallas, Eugene McDermott Ctr Human Growth & Dev, Dallas, TX 75390 USA
[4] Childrens Med Ctr, Dallas, TX 75235 USA
[5] Barnes Jewish Hosp, Dept Labs, St Louis, MO 63110 USA
[6] Washington Univ, Sch Med, Dept Pediat, Dept Pathol & Immunol, St Louis, MO 63110 USA
[7] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA
[8] Avero Diagnost, Irving, TX USA
关键词
23S RIBOSOMAL-RNA; PEPTIDYL-TRANSFERASE CENTER; GENE; SUSCEPTIBILITY; FAECIUM; UPDATE;
D O I
10.1128/JCM.01929-15
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The objective of this study was to investigate an apparent increase in linezolid-nonsusceptible staphylococci and enterococci following a laboratory change in antimicrobial susceptibility testing from disk diffusion to an automated susceptibility testing system. Isolates with nonsusceptible results (n = 27) from Vitek2 were subjected to a battery of confirmatory testing which included disk diffusion, Microscan broth microdilution, Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution, gradient diffusion (Etest), 23S rRNA gene sequencing, and cfr PCR. Our results show that there is poor correlation between methods and that only 70 to 75% of isolates were confirmed as linezolid resistant with alternative phenotypic testing methods (disk diffusion, Microscan broth microdilution, CLSI broth microdilution, and Etest). 23S rRNA gene sequencing identified mutations previously associated with linezolid resistance in 16 (59.3%) isolates, and the cfr gene was detected in 3 (11.1%) isolates. Mutations located at positions 2576 and 2534 of the 23S rRNA gene were most common. In addition, two previously undescribed variants (at positions 2083 and 2345 of the 23S rRNA gene) were also identified and may contribute to linezolid resistance.
引用
收藏
页码:1289 / 1294
页数:6
相关论文
共 20 条
[1]  
[Anonymous], 2014, Clsi. M100-S24
[2]  
[Anonymous], 2012, PERFORMANCE STANDARD
[3]   Clinical and microbiological aspects of linezolid resistance mediated by the cfr gene encoding a 23S rRNA methyltransferase [J].
Arias, Cesar A. ;
Vallejo, Martha ;
Reyes, Jinnethe ;
Panesso, Diana ;
Moreno, Jaime ;
Castaneda, Elizabeth ;
Villegas, Maria V. ;
Murray, Barbara E. ;
Quinn, John P. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (03) :892-896
[4]  
Clinical and Laboratory Standards Institute, 2015, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically Approved Standard, V3rd ed.
[5]  
Grove TL, 2013, NAT CHEM BIOL, V9, P422, DOI [10.1038/NCHEMBIO.1251, 10.1038/nchembio.1251]
[6]   Update of dalbavancin spectrum and potency in the USA: report from the SENTRY Antimicrobial Surveillance Program (2011) [J].
Jones, Ronald N. ;
Sader, Hello S. ;
Flamm, Robert K. .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2013, 75 (03) :304-307
[7]   A new mechanism for chloramphenicol, florfenicol and clindamycin resistance: methylation of 23S ribosomal RNA at A2503 [J].
Kehrenberg, C ;
Schwarz, S ;
Jacobsen, L ;
Hansen, LH ;
Vester, B .
MOLECULAR MICROBIOLOGY, 2005, 57 (04) :1064-1073
[8]   Linezolid in vitro:: mechanism and antibacterial spectrum [J].
Livermore, DM .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2003, 51 :9-16
[9]   Mutations in Ribosomal Protein L3 Are Associated with Oxazolidinone Resistance in Staphylococci of Clinical Origin [J].
Locke, Jeffrey B. ;
Hilgers, Mark ;
Shaw, Karen Joy .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (12) :5275-5278
[10]   Mutations in 23S rRNA at the Peptidyl Transferase Center and Their Relationship to Linezolid Binding and Cross-Resistance [J].
Long, Katherine S. ;
Munck, Christian ;
Andersen, Theis M. B. ;
Schaub, Maria A. ;
Hobbie, Sven N. ;
Boettger, Erik C. ;
Vester, Birte .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (11) :4705-4713