Plasmodium berghei-infected primary hepatocytes process and present the circumsporozoite protein to specific CD8+ T cells in vitro

被引:73
作者
Bongfen, Silayuv E.
Torgler, Ralph
Romero, Jackeline F.
Renia, Laurent
Corradin, Giampietro
机构
[1] Univ Lausanne, Dept Biochem, Epalinges, Switzerland
[2] Hop Cochin, Dept Immunol, Inst Cochin, F-75674 Paris, France
[3] INSERM, Paris, France
[4] CNRS, UMR, Paris, France
[5] Univ Paris 05, Paris, France
关键词
D O I
10.4049/jimmunol.178.11.7054
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A substantial and protective response against malaria liver stages is directed against the circumsporozoite protein (CSP) and involves induction of CD8(+) T cells and production of IFN-gamma. CSP-derived peptides have been shown to be presented on the surface of infected hepatocytes in the context of MHC class I molecules. However, little is known about how the CSP and other sporozoite Ags are processed and presented to CD8(+) T cells. We investigated how primary hepatocytes from BALB/c mice process the CSP of Plasmodium berghei after live sporozoite infection and present CSP-derived peptides to specific H-2K(d)-restricted CD8(+) T cells in vitro. Using both wild-type and spect(-/-) P. berghei sporozoites, we show that both infected and traversed primary hepatocytes process and present the CSP. The processing and presentation pathway was found to involve the proteasome, Ag transport through a postendoplasmic reticulum compartment, and aspartic proteases. Thus, it can be hypothesized that infected hepatocytes can contribute in vivo to the elicitation and expansion of a T cell response.
引用
收藏
页码:7054 / 7063
页数:10
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