Characterization of microsatellite markers in Fagus sylvatica L. and Fagus orientalis Lipsky

被引：87
|
作者：
Pastorelli, R
Smulders, MJM
Van't Westende, WPC
Vosman, B
Giannini, R
Vettori, C
Vendramin, GG
机构：
[1] CNR, Ist Genet Vegetale, I-50134 Florence, Italy
[2] Plant Res Int, Dept Biodivers, Wageningen, Netherlands
来源：
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期
关键词：
Fagus; genetic diversity; inheritance; SSR;
D O I：
10.1046/j.1471-8286.2003.00355.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Using an enrichment procedure, we cloned microsatellite repeats from European beech (Fagus sylvatica U and developed primers for the amplification of microsatellite markers. Six polymorphic loci were characterized which produced 3-21 alleles in 70 individuals from one Italian population, with an observed heterozygosity between 0.58 and 0.85. All six loci amplified fragments which were polymorphic in the closely related species, Fagus orientalis, also. Owing to their very high degree of variation, these markers should be very useful in gene flow studies of these species.
引用
收藏
页码：76 / 78
页数：3
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