Insulin and IR-β in pig spermatozoa: a role of the hormone in the acquisition of fertilizing ability

被引:30
作者
Carpino, A. [1 ]
Rago, V. [1 ]
Guido, C. [2 ,3 ]
Casaburi, I. [2 ,3 ]
Aquila, S. [2 ,3 ]
机构
[1] Univ Calabria, Dept Cell Biol, I-87030 Cosenza, Italy
[2] Univ Calabria, Dept Pharmacobiol, Fac Pharm, I-87030 Cosenza, Italy
[3] Univ Calabria, Ctr Sanit, I-87030 Cosenza, Italy
来源
INTERNATIONAL JOURNAL OF ANDROLOGY | 2010年 / 33卷 / 03期
关键词
fertility; insulin; insulin receptor beta; pig spermatozoa; reproduction; EXOGENOUS INSULIN; ACROSOME REACTION; SECRETION; RECEPTOR; LEPTIN; MEN; RNA; CAPACITATION; ENERGY; CELLS;
D O I
10.1111/j.1365-2605.2009.00971.x
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
P>Recent studies have revealed that insulin, the main regulator of the glucose homeostasis in somatic cells, is expressed in human spermatozoa which are also able to secrete it. This study investigated the expression of insulin and insulin receptor beta in pig spermatozoa, at immunohistochemical protein and mRNA level. The immunofluorescence assay revealed that insulin and its receptor were co-localized in the sperm midpiece, while insulin was also detected in the acrosomal region. Western blot evidenced a 36 kDa band for insulin and a 95 kDa band for insulin receptor, such as reported in somatic cells. In addition, both insulin and insulin receptor transcripts were detected in pig spermatozoa. Interestingly, a possible biological role of the hormone was evidenced during pig sperm capacitation and acrosome reaction. In fact, the results showed that insulin (0.01 and 0.1 nm) can induce both the activities. A possible autocrine short loop of insulin in pig spermatozoa was suggested by the evaluation of the hormone secretion in both uncapacitated and capacitated spermatozoa. Furthermore, spontaneous sperm capacitation and acrosome reaction were stimulated by glucose and inhibited by the blockage of insuline release (nifedipine). In conclusion, this work has firstly demonstrated the expression of insulin and of its receptor, as well as the insulin secretion by pig spermatozoa, thereby suggesting an unexpected significance of the hormone in the acquisition of the male gamete fertilizing ability.
引用
收藏
页码:554 / 562
页数:9
相关论文
共 36 条
[1]   INSULIN-LIKE GROWTH-FACTORS AS INTRAOVARIAN REGULATORS OF GRANULOSA-CELL GROWTH AND FUNCTION [J].
ADASHI, EY ;
RESNICK, CE ;
DERCOLE, AJ ;
SVOBODA, ME ;
VANWYK, JJ .
ENDOCRINE REVIEWS, 1985, 6 (03) :400-420
[2]   Increased concentrations of the oxidative DNA adduct 7,8-dihydro-8-oxo-2-deoxyguanosine in the germ-line of men with type 1 diabetes [J].
Agbaje, I. M. ;
McVicar, C. M. ;
Schock, B. C. ;
McClure, N. ;
Atkinson, A. B. ;
Rogers, D. ;
Lewis, S. E. M. .
REPRODUCTIVE BIOMEDICINE ONLINE, 2008, 16 (03) :401-409
[3]   Insulin dependant diabetes mellitus: implications for male reproductive function [J].
Agbaje, I. M. ;
Rogers, D. A. ;
McVicar, C. M. ;
McClure, N. ;
Atkinson, A. B. ;
Mallidis, C. ;
Lewis, S. E. M. .
HUMAN REPRODUCTION, 2007, 22 (07) :1871-1877
[4]   HYBRID INSULIN GENES REVEAL A DEVELOPMENTAL LINEAGE FOR PANCREATIC ENDOCRINE-CELLS AND IMPLY A RELATIONSHIP WITH NEURONS [J].
ALPERT, S ;
HANAHAN, D ;
TEITELMAN, G .
CELL, 1988, 53 (02) :295-308
[5]   Diabetes and the Impairment of Reproductive Function: Possible Role of Mitochondria and Reactive Oxygen Species [J].
Amaral, Sandra ;
Oliveira, Paulo J. ;
Ramalho-Santos, Joao .
CURRENT DIABETES REVIEWS, 2008, 4 (01) :46-54
[6]   Arguments raised by the recent discovery that insulin and leptin are expressed in and secreted by human ejaculated spermatozoa [J].
Andò, S ;
Aquila, S .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2005, 245 (1-2) :1-6
[7]   Autocrine regulation of insulin secretion in human ejaculated spermatozoa [J].
Aquila, S ;
Gentile, M ;
Middea, E ;
Catalano, S ;
Andò, S .
ENDOCRINOLOGY, 2005, 146 (02) :552-557
[8]   Human ejaculated spermatozoa contain active P450 aromatase [J].
Aquila, S ;
Sisci, D ;
Gentile, M ;
Middea, E ;
Siciliano, L ;
Andò, S .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2002, 87 (07) :3385-3390
[9]   Leptin and leptin receptor in pig spermatozoa: evidence of their involvement in sperm capacitation and survival [J].
Aquila, Saveria ;
Rago, Vittoria ;
Guido, Carmela ;
Casaburi, Ivan ;
Zupo, Silvia ;
Carpino, Amalia .
REPRODUCTION, 2008, 136 (01) :23-32
[10]   Insulin-stimulated insulin secretion in single pancreatic beta cells [J].
Aspinwall, CA ;
Lakey, JRT ;
Kennedy, RT .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (10) :6360-6365