Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste

被引：94
|
作者：
Songsiriritthigul, Chomphunuch [2 ]
Lapboonrueng, Sasithorn [1 ,2 ]
Pechsrichuang, Phornsiri [1 ]
Pesatcha, Puntarika [1 ]
Yamabhai, Montarop [1 ]
机构：
[1] Suranaree Univ Technol, Inst Agr Technol, Sch Biotechnol, Nakhon Ratchasima 30000, Thailand
[2] Synchrotron Light Res Inst Publ Org, Nakhon Ratchasima 30000, Thailand
来源：
BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 11期
关键词：
Chitinase; Bacillus licheniformis; Bioconversion; Chitin; Waste; COMPLETE GENOME SEQUENCE; MARINE SHELLFISH WASTES; THERMOSTABLE CHITINASE; ENZYMATIC DEGRADATION; BIOACTIVE MOLECULES; ENZYMES; PURIFICATION; PROCESSIVITY; GLUCOSAMINE; SECRETION;
D O I：
10.1016/j.biortech.2010.01.036
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Chitinase (EC 3.2.1.14) is an enzyme with multiple industrial applications. These include bioconversion of chitin waste, a highly resistant and abundant biopolymer from crustacean food industry, into glucosamine and chito-oligosaccharide value-added products. This paper reports on the expression of endochitinase (ChiA) from Bacillus licheniformis strain DSM8785 in E. coli and characterization of the recombinant enzyme. Recombinant ChiA could efficiently convert colloidal chitin to N-acetyl glucosamine and chitobiose at pH 4.0, 6.0 and 9.0 at 50 degrees C and retained its activity up to 3 days under these conditions, suggesting that this enzyme is suitable for bioconversion of chitin waste. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：4096 / 4103
页数：8
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