Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste

被引：94
|
作者：
Songsiriritthigul, Chomphunuch [2 ]
Lapboonrueng, Sasithorn [1 ,2 ]
Pechsrichuang, Phornsiri [1 ]
Pesatcha, Puntarika [1 ]
Yamabhai, Montarop [1 ]
机构：
[1] Suranaree Univ Technol, Inst Agr Technol, Sch Biotechnol, Nakhon Ratchasima 30000, Thailand
[2] Synchrotron Light Res Inst Publ Org, Nakhon Ratchasima 30000, Thailand
来源：
BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 11期
关键词：
Chitinase; Bacillus licheniformis; Bioconversion; Chitin; Waste; COMPLETE GENOME SEQUENCE; MARINE SHELLFISH WASTES; THERMOSTABLE CHITINASE; ENZYMATIC DEGRADATION; BIOACTIVE MOLECULES; ENZYMES; PURIFICATION; PROCESSIVITY; GLUCOSAMINE; SECRETION;
D O I：
10.1016/j.biortech.2010.01.036
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Chitinase (EC 3.2.1.14) is an enzyme with multiple industrial applications. These include bioconversion of chitin waste, a highly resistant and abundant biopolymer from crustacean food industry, into glucosamine and chito-oligosaccharide value-added products. This paper reports on the expression of endochitinase (ChiA) from Bacillus licheniformis strain DSM8785 in E. coli and characterization of the recombinant enzyme. Recombinant ChiA could efficiently convert colloidal chitin to N-acetyl glucosamine and chitobiose at pH 4.0, 6.0 and 9.0 at 50 degrees C and retained its activity up to 3 days under these conditions, suggesting that this enzyme is suitable for bioconversion of chitin waste. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：4096 / 4103
页数：8
相关论文
共 50 条
  • [1] Molecular characterisation of Bacillus chitinase for bioconversion of chitin waste
    Saleem, Faiza
    Nisar, Uzma
    Younas, Aisha
    Jabeen, Faiza
    Qazi, Javed Iqbal
    Khursheed, Nazia
    Munir, Neelma
    Naz, Shagufta
    Shakoori, Abdul Rauf
    NATURAL PRODUCT RESEARCH, 2016, 30 (06) : 720 - 723
  • [2] Marine Bacillus haynesii chitinase: Purification, characterization and antifungal potential for sustainable chitin bioconversion
    Govindaraj, Vishnupriya
    Kim, Se -Kwon
    Raval, Ritu
    Raval, Keyur
    CARBOHYDRATE RESEARCH, 2024, 541
  • [3] Bioconversion of chitin waste using a cold-adapted chitinase to produce chitin oligosaccharides
    Fu, Xing
    Guo, Yunxue
    Jin, Yongguo
    Ma, Meihu
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2020, 133
  • [4] Isolation and molecular characterization of chitinase-deficient Bacillus licheniformis strains capable of deproteinization of shrimp shell waste to obtain highly viscous chitin
    Waldeck, Jens
    Daum, Gabriele
    Bisping, Bernward
    Meinhardt, Friedhelm
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (12) : 7879 - 7885
  • [5] Bioconversion of feather waste into bioactive nutrients in water by Bacillus licheniformis WHU
    Jing Zhang
    Manyu Liang
    Lijuan Wu
    Yanhong Yang
    Yingjie Sun
    Qin Wang
    Xiaowei Gao
    Applied Microbiology and Biotechnology, 2023, 107 : 7055 - 7070
  • [6] Characterization of a Bacillus thuringiensis chitinase that binds to cellulose and chitin
    Honda, Shotaro
    Kunii, Toshiyuki
    Nohara, Kenta
    Wakita, Satoshi
    Sugahara, Yasusato
    Kawakita, Masao
    Oyama, Fumitaka
    Sakaguchi, Masayoshi
    AMB EXPRESS, 2017, 7
  • [7] Characterization of a Bacillus thuringiensis chitinase that binds to cellulose and chitin
    Shotaro Honda
    Toshiyuki Kunii
    Kenta Nohara
    Satoshi Wakita
    Yasusato Sugahara
    Masao Kawakita
    Fumitaka Oyama
    Masayoshi Sakaguchi
    AMB Express, 7
  • [8] Bioconversion of feather waste into bioactive nutrients in water by Bacillus licheniformis WHU
    Zhang, Jing
    Liang, Manyu
    Wu, Lijuan
    Yang, Yanhong
    Sun, Yingjie
    Wang, Qin
    Gao, Xiaowei
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2023, 107 (23) : 7055 - 7070
  • [9] Chitinase of Bacillus licheniformis from oyster shell as a probe to detect chitin in marine shells
    Shah Md. Asraful Islam
    Kye Man Cho
    Sun Joo Hong
    Renukaradhya K. Math
    Jong Min Kim
    Myoung Geun Yun
    Ji Joong Cho
    Jae Young Heo
    Young Han Lee
    Hoon Kim
    Han Dae Yun
    Applied Microbiology and Biotechnology, 2010, 86 : 119 - 129
  • [10] Chitinase of Bacillus licheniformis from oyster shell as a probe to detect chitin in marine shells
    Islam, Shah Md. Asraful
    Cho, Kye Man
    Hong, Sun Joo
    Math, Renukaradhya K.
    Kim, Jong Min
    Yun, Myoung Geun
    Cho, Ji Joong
    Heo, Jae Young
    Lee, Young Han
    Kim, Hoon
    Yun, Han Dae
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 86 (01) : 119 - 129
12345