Fractionation and characterization of mushroom dietary fiber (nonstarch polysaccharides) as potential nutraceuticals from sclerotia of Pleurotus tuber-regium (Fries) Singer

被引：39

作者：

Cheung, PCK ^{[1
]}

Lee, MY ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 08期

关键词：

mushroom sclerotium; nonstarch polysaccharides; Pleurotus tuber-regium; structure;

D O I：

10.1021/jf000382s

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The nonstarch polysaccharides (NSPs) in the total dietary fiber (TDF) from the sclerotia of Pleurotus tuber-regium (tiger milk mushroom) were fractionated by the sequential use of chemical solvents. About half of the TDF was solubilized and two major alkali-soluble fractions (1 and 4 N sodium hydroxide) that contained 126 and 293 g/kg TDF were obtained. Sugar analysis and infrared spectroscopy indicated that the NSPs in these alkali-soluble fractions were mainly beta-glucans and chitin. These alkali-soluble NSPs were further purified by anion-exchange chromatography followed by gel permeation chromatographic separation. Methylation analysis revealed that these purified glucans were highly branched and contained a mixture of sugar linkages of beta-1,3, beta-1,6, and beta-1,4. The potential use of these sclerotial beta-glucans as nutraceuticals was discussed.

引用

页码：3148 / 3151

页数：4

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