TGF-β induces periodontal ligament stem cell senescence through increase of ROS production

被引：31
作者：
Fan, Chun [1 ]
Ji, Qiuxia [1 ]
Zhang, Chunyang [1 ]
Xu, Shuo [1 ]
Sun, Hui [1 ]
Li, Zhiyuan [2 ]
机构：
[1] Qingdao Univ, Dept Periodontol, Affiliated Hosp, 1677 Wutaishan Rd, Qingdao 266555, Shandong, Peoples R China
[2] Qingdao Univ, Key Lab, Affiliated Hosp, Dept Otolaryngol Head & Neck Surg, 1677 Wutaishan Rd, Qingdao 266555, Shandong, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2019年 / 20卷 / 04期
基金：
中国国家自然科学基金;
关键词：
periodontal ligament stem cells; transforming growth factor-beta; cellular senescence; reactive oxygen species; GROWTH-FACTOR-BETA; OXIDATIVE STRESS; REGENERATION; BIOLOGY;
D O I：
10.3892/mmr.2019.10580
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Periodontal ligament stem cells (PDLSCs) are vital for the regeneration of periodontal tissue. Transforming growth factor (TGF) beta 1, a potent stimulator of tissue regeneration, is extensive and abundant in the bone matrix. However, the effect of TGF-beta 1 in periodontal differentiation remains to be elucidated. The present study aimed to evaluate the effect of TGF-beta 1 on human PDLSCs. PDLSCs were isolated using CD146 microbeads, and characterized by flow cytometry. The present study demonstrated that treatment with TGF-beta 1 induced PDLSC senescence, characterized by increases in senescence-associated beta-galactosidase activity and elevation of both p16 and p21 expression. Furthermore, TGF-beta 1 treatment demonstrated the capacity to induce the production of reactive oxygen species (ROS). Of note, addition of a ROS scavenger successfully rescued the TGF-beta 1-induced PDLSC senescence. Thus, the present results indicated that TGF-beta 1 may serve a vital role in PDLSC senescence, and thus represent a potential target involved in the fabrication and formation of hard tissue for clinical treatment.
引用
收藏
页码：3123 / 3130
页数：8
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