High-throughput sequencing reveals a novel closterovirus in arracacha (Arracacia xanthorrhiza)

被引：7

作者：

Orilio, Anelise F. ^{[1
]}

Blawid, Rosana ^{[1
]}

Costa, Gabriela A. ^{[1
]}

Gomes, Suzane S. V. S. F. ^{[1
]}

Nagata, Tatsuya ^{[1
]}

Madeira, Nuno R. ^{[2
]}

Inoue-Nagata, Alice K. ^{[2
]}

Resende, Renato O. ^{[1
]}

机构：

[1] Univ Brasilia, Dept Cell Biol, Campus Univ Darcy Ribeiro, BR-70910900 Brasilia, DF, Brazil

[2] Embrapa Vegetables, Caixa Postal 218, BR-70275970 Brasilia, DF, Brazil

来源：

ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 09期

关键词：

COMPLETE GENOME SEQUENCE;

D O I：

10.1007/s00705-018-3863-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

High-throughput sequencing analysis detected a clostero-like virus from arracacha plants (Arracacia xanthorrhiza) in Brazil. The complete genome sequence, confirmed by RACE and Sanger sequencing, consists of 15,763 nucleotides with nine predicted open reading frames (ORFs) in a typical closterovirus genome organisation. The putative RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homologue (Hsp70h), and coat protein showed 55-65, 38-44, and 20-36% amino acid sequence identity, respectively, to the homologous proteins of known closteroviruses. Phylogenetic analysis of Hsp70h showed that this putative novel arracacha plant virus was related to members of the genus Closterovirus in the family Closteroviridae. These results suggest that this virus, tentatively named "arracacha virus 1" (AV-1), is a novel member of the genus Closterovirus. This is the first closterovirus identified in arracacha plants.

引用

页码：2547 / 2550

页数：4