Multi-gene engineering in plants with RNA-guided Cas9 nuclease

被引：26

作者：

Raitskin, Oleg ^{[1
]}

Patron, Nicola J. ^{[1
]}

机构：

[1] Sainsbury Lab, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2016年 / 37卷

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

CRISPR/CAS9-MEDIATED TARGETED MUTAGENESIS; GENE-EXPRESSION; PAIRED NICKASES; HUMAN-CELLS; GENOME; CRISPR; SYSTEM; ARABIDOPSIS; MULTIPLEX; RICE;

D O I：

10.1016/j.copbio.2015.11.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of RNA-guided Cas9 endonuclease for the concurrent engineering of multiple genes has been demonstrated in a number of plant species. Although Cas9 is a large monomeric protein, the single guide RNA (sgRNA) that directs it to a specific DNA target sequence is small and easy to reprogram. It is therefore relatively simple to produce numerous sgRNAs to target multiple endogenous sequences. Several approaches to express multiple sgRNAs and Cas9 in plants for the purpose of simultaneous editing or transcriptional regulation of many genes have recently been reported.

引用

页码：69 / 75

页数：7

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