Preparative isolation and purification of geniposide from gardenia fruits by centrifugal partition chromatography

被引：31

作者：

Kim, Chul Young

Kim, Jinwoong ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Pharm, Seoul 151742, South Korea

[2] Seoul Natl Univ, Pharmaceut Sci Res Inst, Seoul 151742, South Korea

来源：

PHYTOCHEMICAL ANALYSIS | 2007年 / 18卷 / 02期

关键词：

centrifugal partition chromatography; iridoid glycoside; geniposide; Gardenia jasminoides; Rubiaceae;

D O I：

10.1002/pca.958

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The iridoid glycoside, geniposide was purified by centrifugal partition chromatography (CPC) with a two-phase solvent system composed of ethyl acetate:isopropanol:water (3:2:5, v/v) from an 80% methanolic extract of fruits of Gardenia jusminoides. Preparative CPC yielded 56.2 mg of geniposide in a one-step separation of 500 mg of extract, with a purity of 95% as determined by HPLC. Isolated gemposide was identified from its H-1-NMR, C-13-NMR and MS spectra. Copyright (c) 2007 John Wiley & Sons, Ltd.

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收藏

页码：115 / 117

页数：3

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