Single-Molecule and Superresolution Imaging in Live Bacteria Cells

被引：33

作者：

Biteen, Julie S. ^{[1
,2
]}

Moerner, W. E. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY | 2010年 / 2卷 / 03期

关键词：

OPTICAL RECONSTRUCTION MICROSCOPY; FLUORESCENCE SPECTROSCOPY; LOCALIZATION MICROSCOPY; TRANSLATIONAL DIFFUSION; CAULOBACTER-CRESCENTUS; DIFFRACTION-LIMIT; PLASMA-MEMBRANE; PROTEIN; POLARITY; PROBES;

D O I：

10.1101/cshperspect.a000448

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Single-molecule imaging enables biophysical measurements devoid of ensemble averaging, gives enhanced spatial resolution beyond the diffraction limit, and permits superresolution reconstructions. Here, single-molecule and superresolution imaging are applied to the study of proteins in live Caulobacter crescentus cells to illustrate the power of these methods in bacterial imaging. Based on these techniques, the diffusion coefficient and dynamics of the histidine protein kinase PleC, the localization behavior of the polar protein PopZ, and the treadmilling behavior and protein superstructure of the structural protein MreB are investigated with sub-40-nm spatial resolution, all in live cells.

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页数：14

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