Sensitive and rapid aptasensing of chloramphenicol by colorimetric signal transduction with a DNAzyme-functionalized gold nanoprobe

被引：43

作者：

Huang, Wan ^{[1
]}

Zhang, Hongyu ^{[1
]}

Lai, Guosong ^{[1
]}

Liu, Shun ^{[1
]}

Li, Bo ^{[1
]}

Yu, Aimin ^{[1
,2
]}

机构：

[1] Hubei Normal Univ, Dept Chem, Hubei Key Lab Pollutant Anal & Reuse Technol, Huangshi 435002, Peoples R China

[2] Swinbume Univ Technol, Fac Sci Engn & Technol, Dept Chem & Biotechnol, Hawthorn, Vic 3122, Australia

来源：

FOOD CHEMISTRY | 2019年 / 270卷

基金：

中国国家自然科学基金;

关键词：

Aptasensor; Chloramphenicol; Colorimetry; DNAzyme; Food analysis; DOUBLE-STRANDED DNA; ULTRASENSITIVE DETECTION; ANTIBIOTIC-RESIDUES; BLOOD-SERUM; AMPLIFICATION; ASSAY; NANOPARTICLES; MILK; STREPTOMYCIN; GRAPHENE;

D O I：

10.1016/j.foodchem.2018.07.127

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

By combination of the aptamer biorecognition with the colorimetric signal transduction of a DNAzyme-functionalized nanoprobe, a new biosensing method was developed for the rapid and sensitive detection of chloramphenicol (CAP). The nanoprobe was prepared through the functionalization of gold nanoparticles with the complementary oligonucleotide against aptamer and high-content hemin/G-quadruplex DNAzyme. When one-step incubating the nanoprobe and CAP at a constructed aptamer-magnetic bead (MB) biosensing platform, due to the competitive biorecognition reaction, the nanoprobes related with CAP amounts were quantitative captured onto the MB surface. Based on the catalytic reaction of the peroxidase-mimicking DNAzyme, a colored substance was produced for the colorimetric signal transduction of the method. Due to the great signal amplification of the nanoprobe, a very low detection limit down to 0.13 pg/mL was obtained. Considering the excellent performance of the aptasensing method and satisfactory results for milk sample experiments, it indicates good reliability for practical applications.

引用

页码：287 / 292

页数：6

共 40 条

[1] A novel colorimetric sandwich aptasensor based on an indirect competitive enzyme-free method for ultrasensitive detection of chloramphenicol [J].