Characterization of Fowl adenovirus serotype 4 isolated from chickens with hydropericardium syndrome based on analysis of the short fiber protein gene

被引:49
|
作者
Mase, Masaji [1 ]
Nakamura, Kikuyasu [1 ]
Imada, Tadao [1 ]
机构
[1] Natl Inst Anim Hlth, Res Team Zoonot Dis, Tsukuba, Ibaraki 3050856, Japan
关键词
Fowl adenovirus; hydropericardium syndrome; phylogenetic analysis; INFECTIOUS HYDROPERICARDIUM; NUCLEOTIDE-SEQUENCE; BROILER-CHICKENS; DNA-SEQUENCE; OUTBREAKS; CELO;
D O I
10.1177/104063871002200207
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The sequences of short fiber genes of the Foul adenovirus serotype 4 (FAdV-4), including isolates from chickens with hydropericardium syndrome (HPS), in Japan, India, and Pakistan were compared. By phylogenetic analysis based on complete nucleotide sequences of this gene, FAdV-4 strains from HPS (HPS FAdV-4) in Japan. India, and Pakistan fell into a different cluster from FAdV-4 strains not derived from HPS. Hydropericardium syndrome FAdV-4 isolates were differentiated from other FAdV-4 strains by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis using the enzyme the Alu I. The use of PCR-RFLP analysis of short Fiber genes may be useful to distinguish among FAdV-4 strains.
引用
收藏
页码:218 / 223
页数:6
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