AN IMPROVED METHOD FOR in vitro REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.)

In vitro regeneration of common bean (Phaseolus vulgaris L.) is a requirement for genetic transformation which involves induction and development to the whole plant. Success in regeneration of common bean front tissue organ culture has been achieved to sonic extent in the last years. However, genotype effects in regeneration response as well as efficiency and reproducibility have been a limiting factor. Embryos, from four different varieties, were excised from sterilized mature seeds and cultured in Murashige and Skoog (MS) or Gamborg (GM) media containing 6-benzylaminopurine (BAP) (10 mg(-1)) and adenine (A) (0 or 20 mg(-1)). Efficient regeneration was achieved when inducing formation of differentiation of cells like bud clusters at the internodal segment of the embryo axes in the four varieties studied. One way analysis of variance and Tukey media comparison (p <= 0.05) showed that regeneration efficiency varied considerably between the two basic media. GM media provided high bud cluster formation (97.8 to 100 %) and full plant regeneration (93 %), whereas NIS medium showed lower bud cluster formation (15 to 73 %), and full plant regeneration (29 %). It is provided evidence of how the culture media and growth regulators influence the regeneration of common bean, when seeking for an efficient transformation protocol.