Study of freshly excised brain tissues using terahertz imaging

被引：165

作者：

Oh, Seung Jae ^{[1
]}

Kim, Sang-Hoon ^{[1
]}

Ji, Young Bin ^{[2
]}

Jeong, Kiyoung ^{[3
]}

Park, Yeonji ^{[1
]}

Yang, Jaemoon ^{[4
]}

Park, Dong Woo ^{[5
]}

Noh, Sam Kyu ^{[4
,5
]}

Kang, Seok-Gu ^{[6
]}

Huh, Yong-Min ^{[1
]}

Son, Joo-Hiuk ^{[7
]}

Suh, Jin-Suck ^{[1
,4
]}

机构：

[1] Yonsei Univ, Coll Med, YUMS KRIBB Med Convergence Res Inst, Seoul 120752, South Korea

[2] Yonsei Univ, Yonsei Inst Convergence Technol, Seoul 120752, South Korea

[3] Yonsei Univ, Nanomed Natl Core Res Ctr, Seoul 120749, South Korea

[4] Yonsei Univ, Coll Med, Dept Radiol, Seoul 120752, South Korea

[5] Korea Res Inst Stand & Sci, Nano Mat Evaluat Ctr, Taejon 305340, South Korea

[6] Yonsei Univ, Severance Hosp, Coll Med, Dept Neurosurg, Seoul 120752, South Korea

[7] Univ Seoul, Dept Phys, Seoul 130743, South Korea

来源：

BIOMEDICAL OPTICS EXPRESS | 2014年 / 5卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

QUANTUM CASCADE LASER; SPECTROSCOPY; CONTRAST; GLIOMA; TUMORS;

D O I：

10.1364/BOE.5.002837

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrated that tumors in freshly excised whole brain tissue could be differentiated clearly from normal brain tissue using a reflection-type terahertz (THz) imaging system. THz binary images of brain tissues with tumors indicated that the tumor boundaries in the THz images corresponded well to those in visible images. Grey and white-matter regions were distinguishable owing to the different distribution of myelin in the brain tissue. THz images corresponded closely with magnetic resonance imaging (MRI) results. The MRI and hematoxylin and eosin-stained microscopic images were investigated to account for the intensity differences in the THz images for fresh and paraffin-embedded brain tissue. Our results indicated that the THz signals corresponded to the cell density when water was removed. Thus, THz imaging could be used as a tool for label-free and real-time imaging of brain tumors, which would be helpful for physicians to determine tumor margins during brain surgery. (C)2014 Optical Society of America

引用

页码：2837 / 2842

页数：6

共 20 条

[1] Terahertz pulse imaging: A pilot study of potential applications in dentistry [J].

Crawley, DA ;

Longbottom, C ;

Cole, BE ;

Ciesla, CM ;

Arnone, D ;

Wallace, VP ;

Pepper, M .

CARIES RESEARCH, 2003, 37 (05) :352-359

[2] Imaging with a Terahertz quantum cascade laser [J].

Darmo, J ;

Tamosiunas, V ;

Fasching, G ;

Kröll, J ;

Unterrainer, K ;

Beck, M ;

Giovannini, M ;

Faist, J ;

Kremser, C ;

Debbage, P .

OPTICS EXPRESS, 2004, 12 (09) :1879-1884

[3]

Edelman R. R., 1996, CLIN MAGNETIC RESONA

[4] Terahertz pulsed imaging of human breast tumors [J].

Fitzgerald, AJ ;

Wallace, VP ;

Jimenez-Linan, M ;

Bobrow, L ;

Pye, RJ ;

Purushotham, AD ;

Arnone, DD .

RADIOLOGY, 2006, 239 (02) :533-540

[5] Fluorescence-Guided Surgery for Malignant Glioma: A Review on Aminolevulinic Acid Induced Protoporphyrin IX Photodynamic Diagnostic in Brain Tumors [J].

Hefti, Martin ;

Mehdorn, H. Maximilian ;

Albert, Ina ;

Doerner, Lutz .

CURRENT MEDICAL IMAGING, 2010, 6 (04) :254-258

[6] Terahertz dynamic imaging of skin drug absorption [J].

Kim, Kyung Won ;

Kim, Kwang-Sung ;

Kim, Hyeongmun ;

Lee, Sang Hun ;

Park, Jae-Hak ;

Han, Ju-Hee ;

Seok, Seung-Hyeok ;

Park, Jisuk ;

Choi, YoonSeok ;

Kim, Young Il ;

Han, Joon Koo ;

Son, Joo-Hiuk .

OPTICS EXPRESS, 2012, 20 (09) :9476-9484

[7] Biomedical terahertz imaging with a quantum cascade laser [J].

Kim, SM ;

Hatami, F ;

Harris, JS ;

Kurian, AW ;

Ford, J ;

King, D ;

Scalari, G ;

Giovannini, M ;

Hoyler, N ;

Faist, J ;

Harris, G .

APPLIED PHYSICS LETTERS, 2006, 88 (15)

[8] Ionic contrast terahertz near-field imaging of axonal water fluxes [J].

Masson, JB ;

Sauviat, MP ;

Martin, JL ;

Gallot, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (13) :4808-4812

[9] Cell hydration as the primary factor in carcinogenesis: A unifying concept [J].

McIntyre, GI .

MEDICAL HYPOTHESES, 2006, 66 (03) :518-526

[10] High-Field ioMRI in Glioblastoma Surgery: Improvement of Resection Radicality and Survival for the Patient? [J].

Mehdorn, H. Maximilian ;

Schwartz, Felix ;

Dawirs, Stefan ;

Hedderich, Jurgen ;

Doerner, Lutz ;

Nabavi, Arya .

INTRAOPERATIVE IMAGING, 2011, 109 :103-106

← 1 2 →