1H-13C separated local field spectroscopy of uniformly 13C labeled peptides and proteins

被引：7

作者：

Lin, Eugene C. ^{[1
]}

Wu, Chin H. ^{[1
]}

Yang, Yuan ^{[1
]}

Grant, Christopher V. ^{[1
]}

Opella, Stanley J. ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

JOURNAL OF MAGNETIC RESONANCE | 2010年 / 206卷 / 01期

基金：

美国国家卫生研究院;

关键词：

C-13; NMR; PISEMO; Solid-state NMR; Oriented samples; SOLID-STATE NMR; NUCLEAR-MAGNETIC-RESONANCE; ALIGNED SAMPLES; RESOLUTION; PROBE; N-15; ASSIGNMENT; SPECTRA;

D O I：

10.1016/j.jmr.2010.06.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

By incorporating homonuclear decoupling on both the H-1 and C-13 channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100% labeled with C-13. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated Observation) can be performed as a conventional two-dimensional experiment, or with windowed detection as a one-dimensional experiment that offers flexibility as a building block for shiftless and other multidimensional triple-resonance experiments with the inclusion of N-15 irradiation. The triple-resonance MAGC probe used to perform these experiments at 500 MHz is described. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：105 / 111

页数：7

共 31 条

[1] PROTEIN-STRUCTURE BY SOLID-STATE NMR [J].

CROSS, TA ;

OPELLA, SJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (02) :306-308

[2] STRATEGY FOR NITROGEN NMR OF BIO-POLYMERS [J].

CROSS, TA ;

DIVERDI, JA ;

OPELLA, SJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (06) :1759-1761

[3] SINGLE COIL PROBE WITH TRANSMISSION-LINE TUNING FOR NUCLEAR MAGNETIC DOUBLE-RESONANCE [J].

CROSS, VR ;

HESTER, RK ;

WAUGH, JS .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1976, 47 (12) :1486-1488

[4] A MULTI-NUCLEAR DOUBLE-TUNED PROBE FOR APPLICATIONS WITH SOLIDS OR LIQUIDS UTILIZING LUMPED TUNING ELEMENTS [J].

DOTY, FD ;

INNERS, RR ;

ELLIS, PD .

JOURNAL OF MAGNETIC RESONANCE, 1981, 43 (03) :399-416

[5] NUCLEAR MAGNETIC RESONANCE LINE NARROWING BY A ROTATING RF FIELD [J].

GOLDBURG, WI ;

LEE, M .

PHYSICAL REVIEW LETTERS, 1963, 11 (06) :255-&

[6] Probes for high field solid-state NMR of lossy biological samples [J].

Grant, Christopher V. ;

Wu, Chin H. ;

Opella, Stanley J. .

JOURNAL OF MAGNETIC RESONANCE, 2010, 204 (02) :180-188

[7] A Modified Alderman-Grant Coil makes possible an efficient cross-coil probe for high field solid-state NMR of lossy biological samples [J].

Grant, Christopher V. ;

Yang, Yuan ;

Glibowicka, Mira ;

Wu, Chin H. ;

Park, Sang Ho ;

Deber, Charles M. ;

Opella, Stanley J. .

JOURNAL OF MAGNETIC RESONANCE, 2009, 201 (01) :87-92

[8] Three-dimensional 13C shift/1H-15N Coupling/15N shift solid-state NMR correlation spectroscopy [J].

Gu, ZT ;

Opella, SJ .

JOURNAL OF MAGNETIC RESONANCE, 1999, 138 (02) :193-198

[9] Two- and three-dimensional 1H/13C PISEMA experiments and their application to backbone and side chain sites of amino acids and peptides [J].

Gu, ZTT ;

Opella, SJ .

JOURNAL OF MAGNETIC RESONANCE, 1999, 140 (02) :340-346

[10] COHERENT AVERAGING EFFECTS IN MAGNETIC RESONANCE [J].

HAEBERLEN, U ;

WAUGH, JS .

PHYSICAL REVIEW, 1968, 175 (02) :453-+

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