Objective of this study was to noninvasively extract quantitative estimates of heart tissue absorption before and after lesion formation. To extract tissue absorption, ultrasound temperature maps were reconstructed from single transmit focus (STF) images acquired with a 3.5MHz Dual-Mode Ultrasound Array (DMUA). Initial heating rate is estimated before and after therapeutic lesion formation through a delivery of a subtherapeutic intensity shot in ex vivo bovine heart tissue. Changes in heating rate are documented for a subset of five subtherapeutic shot intensities. Additionally, repeatability is demonstrated for before and after measurement of the initial heating rate. Careful gross examination of tissue was performed for every location where an absorption measurement was made.