Characterization of single nucleotide polymorphisms for a forward genetics approach using genetic crosses in C57BL/6 and BALB/c substrains of mice

被引：2

作者：

Miura, Ikuo ^{[1
,2
,3
]}

Kikkawa, Yoshiaki ^{[1
,3
]}

Yasuda, Shumpei P. ^{[3
]}

Shinogi, Akiko ^{[2
]}

Usuda, Daiki ^{[2
,4
]}

Kumar, Vivek ^{[5
]}

Takahashi, Joseph S. ^{[6
]}

Tamura, Masaru ^{[2
]}

Masuya, Hiroshi ^{[4
]}

Wakana, Shigeharu ^{[2
,7
]}

机构：

[1] Niigata Univ, Sch Med, Grad Sch Med & Dent Sci, Dept Cooperat Grad Sch,Div Mol Genet,Chuo Ku, 1-757 Asahimachi Dori, Niigata, Niigata 9518510, Japan

[2] RIKEN BioResource Res Ctr, Japan Mouse Clin, Technol & Dev Team Mouse Phenotype Anal, 3-1-1 Koyadai, Tsukuba, Ibaraki 3050074, Japan

[3] Tokyo Metropolitan Inst Med Sci, Dept Basic Med Sci, Deafness Project, Setagaya Ku, 2-1-6 Kamikitazawa, Tokyo 1568506, Japan

[4] RIKEN BioResource Res Ctr, Integrated Bioresource Informat Div, 3-1-1 Koyadai, Tsukuba, Ibaraki 3050074, Japan

[5] Jackson Lab, 600 Main St, Bar Harbor, ME 04609 USA

[6] Univ Texas Southwestern Med Ctr Dallas, Dept Neurosci, 5323 Harry Hines Blvd, Dallas, TX 75390 USA

[7] Fdn Biomed Res & Innovat Kobe, Dept Anim Expt, Creat Lab Innovat Kobe, Chuo Ku, 5F 6-3-7 Minatojima Minamimachi, Kobe, Hyogo 6500047, Japan

来源：

EXPERIMENTAL ANIMALS | 2022年 / 71卷 / 02期

关键词：

BALB/c; C57BL/6; genotyping; single nucleotide polymorphism; substrain; ENU MUTAGENESIS; MUTANT MOUSE; GENOME-WIDE; PHENOTYPES; MODELS; MUTATIONS; DISCOVERY; SEQUENCE; SCREENS; PROGRAM;

D O I：

10.1538/expanim.21-0181

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Forward genetics is a powerful approach based on chromosomal mapping of phenotypes and has successfully led to the discovery of many mouse mutations in genes responsible for various phenotypes. Although crossing between genetically remote strains can produce F2 and backcross mice for chromosomal mapping, the phenotypes are often affected by background effects from the partner strains in genetic crosses. Genetic crosses between substrains might be useful in genetic mapping to avoid genetic background effects. In this study, we investigated single nucleotide polymorphisms (SNPs) available for genetic mapping using substrains of C57BL/6 and BALB/c mice. In C57BL/6 mice, 114 SNP markers were developed and assigned to locations on all chromosomes for full utilization for genetic mapping using genetic crosses between the C57BL/6J and C57BL/6N substrains. Moreover, genetic differences were identified in the 114 SNP markers among the seven C57BL/6 substrains from five production breeders. In addition, 106 SNPs were detected on all chromosomes of BALB/cAJcl and BALB/ cByJJcl substrains. These SNPs could be used for genotyping in BALB/cJ, BALB/cAJcl, BALB/cAnNCrlCrlj, and BALB/cCrSlc mice, and they are particularly useful for genetic mapping using crosses between BALB/cByJJcl and other BALB/c substrains. The SNPs characterized in this study can be utilized for genetic mapping to identify the causative mutations of the phenotypes induced by N-ethyl-N-nitrosourea mutagenesis and the SNPs responsible for phenotypic differences between the substrains of C57BL/6 and BALB/c mice.

引用

页码：240 / 251

页数：12

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