Expression of metabotropic glutamate receptors in murine thymocytes and thymic stromal cells

被引：66

作者：

Storto, M

de Grazia, U

Battaglia, G

Felli, MP

Maroder, M

Gulino, A

Ragona, G

Nicoletti, F

Screpanti, I

Frati, L

Calogero, A ^{[1
]}

机构：

[1] INM Neuromed, Pozzilli, Italy

[2] Univ Aquila, Dept Expt Med, I-67100 Laquila, Italy

[3] Univ Palermo, Dipartimento Biopatol & Metodol Biomed, Palermo, Italy

[4] Univ La Sapienza, Dept Expt Med & Pathol, Rome, Italy

[5] Univ Catania, Dept Pharmacol Sci, Catania, Italy

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2000年 / 109卷 / 02期

关键词：

thymocytes; TC-1S cells; metabotropic glutamate receptors;

D O I：

10.1016/S0165-5728(00)00269-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

RT-PCR combined with immunoblotting showed the expression of group-I (mGlu1 and 5) and group-II (mGlu2? and 3) metabotropic glutamate receptors in whole mouse thymus, isolated thymocytes and TC-1S thymic stromal cell line. Cytofluorimetric analysis showed that mGlu-5 receptors were absent in CD4(-)/CD8(-) but present in more mature CD4(+) CD8(+) and CD4(+)CD8(-) thymocytes. mGlu-1a receptors showed an opposite pattern of expression with respect to mClu5, whereas mGlu2/3 receptor expression did not differ between double negative and double positive cells. mGlu receptors expressed in both thymic cell components were functional, as indicated by measurements of polyphosphoinositide hydrolysis or cAMP formation. These data suggest a possible role for mGlu receptor signalling in the thymus. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：112 / 120

页数：9

共 45 条

[1] INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].