Preparation and evaluation of a polymer-based sulfobetaine zwitterionic stationary phase

被引：15
|
作者：
Liu, Xuefang [1 ]
Jiang, Yu [1 ]
Zhang, Feifang [1 ]
Li, Zongying [1 ]
Yang, Bingcheng [1 ]
机构：
[1] East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, 130 Meilong RD, Shanghai 200237, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2021年 / 1649卷
基金：
中国国家自然科学基金;
关键词：
Hydrophilic interaction liquid chromatography; Zwitterionic stationary phase; Polymer; Saccharide separation; Column bleeding; HYDROPHILIC-INTERACTION CHROMATOGRAPHY; INTERACTION LIQUID-CHROMATOGRAPHY; SEPARATION; SILICA;
D O I：
10.1016/j.chroma.2021.462229
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a polymer-based sulfobetaine zwitterionic stationary phase for hydrophilic interaction liquid chromatography (HILIC). It was prepared by grafting acrylamide-type sulfobetaine monomer instead of common methacrylate-type sulfobetaine onto hydrolysed poly (glycidyl methacrylate-divinylbenzene) (GMA-DVB) microspheres via pendant double bonds of DVB. The phase has been characterized by elemental analysis, scanning electron micrograph and N-2 adsorption-desorption experiment. It shows wider pH tolerance range (from 2 to 12) and excellent separation ability towards common strong polar analytes such as nucleosides and nucleic bases, water-soluble vitamins, amino acids, inorganic anions and cations. Notably, it exhibits negligible baseline noise level (similar to 0.15 pA) under typical HILIC mobile phase. Excellent selectivity in separation of alpha- and beta-anomers of reducing sugars and lactose/lactulose has also been observed. (c) 2021 Elsevier B.V. All rights reserved.
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页数：7
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