Preparation and evaluation of a polymer-based sulfobetaine zwitterionic stationary phase

We describe a polymer-based sulfobetaine zwitterionic stationary phase for hydrophilic interaction liquid chromatography (HILIC). It was prepared by grafting acrylamide-type sulfobetaine monomer instead of common methacrylate-type sulfobetaine onto hydrolysed poly (glycidyl methacrylate-divinylbenzene) (GMA-DVB) microspheres via pendant double bonds of DVB. The phase has been characterized by elemental analysis, scanning electron micrograph and N-2 adsorption-desorption experiment. It shows wider pH tolerance range (from 2 to 12) and excellent separation ability towards common strong polar analytes such as nucleosides and nucleic bases, water-soluble vitamins, amino acids, inorganic anions and cations. Notably, it exhibits negligible baseline noise level (similar to 0.15 pA) under typical HILIC mobile phase. Excellent selectivity in separation of alpha- and beta-anomers of reducing sugars and lactose/lactulose has also been observed. (c) 2021 Elsevier B.V. All rights reserved.