Evaluation of the protective efficacy of a newly identified immunogenic protein, HP0272, of Streptococcus suis

被引：30

作者：

Chen, Bo ^{[1
,2
]}

Zhang, Anding ^{[1
,2
]}

Li, Ran ^{[2
]}

Mu, Xiaofeng ^{[2
]}

He, Hongkui ^{[2
]}

Chen, Huanchun ^{[1
,2
]}

Jin, Meilin ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Unit Anim Infect Dis, Natl Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China

[2] Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China

来源：

FEMS MICROBIOLOGY LETTERS | 2010年 / 307卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Streptococcus suis; HP0272; vaccine candidate; OCCUPATIONAL-EXPOSURE; SURFACE PROTEIN; SEROTYPE-2; PIGS; MENINGITIS; INFECTION; ANTIGEN; INTERLEUKIN-12; EXPRESSION; STRAINS;

D O I：

10.1111/j.1574-6968.2010.01944.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Streptococcus suis serotype 2 (SS2) infection is a major cause of sudden death in pigs and is of concern for humans as it has strong zoonotic capabilities. Developing novel effective vaccines would be beneficial to control SS2 infection. HP0272 is a novel immunogenic surface protein; its protective efficacy remains to be evaluated. The present mouse model found that the purified recombinant HP0272 could elicit a significant humoral antibody response, and to confer complete protection against a lethal dose of SS2 infection. In addition, real-time PCR confirmed that in vivo-induced antigen existed in most SS2 field pathogenic strains, and in half of all reference strains of different serotypes of S. suis. The results indicate that HP0272 has the potential as an effective component of a new vaccine.

引用

页码：12 / 18

页数：7

共 36 条

[1] Intranasal vaccination with pneumococcal surface protein A and interleukin-12 augments antibody-mediated opsonization and protective immunity against Streptococcus pneumoniae infection [J].

Arulanandam, BP ;

Lynch, JM ;

Briles, DE ;

Hollingshead, S ;

Metzger, DW .

INFECTION AND IMMUNITY, 2001, 69 (11) :6718-6724

[2] In vitro phagocytosis and survival of Streptococcus suis capsular type 2 inside murine macrophages [J].

Brazeau, C ;

Gottschalk, M ;

Vincelette, S ;

MartineauDoize, B .

MICROBIOLOGY-UK, 1996, 142 :1231-1237

[3] Streptococcus suis Enolase Functions as a Protective Antigen Displayed on the Bacterial Cell Surface [J].

Feng, Youjun ;

Pan, Xiuzhen ;

Sun, Wen ;

Wang, Changjun ;

Zhang, Huimin ;

Li, Xianfu ;

Ma, Ying ;

Shao, Zhuqing ;

Ge, Junchao ;

Zheng, Feng ;

Gao, George F. ;

Tang, Jiaqi .

JOURNAL OF INFECTIOUS DISEASES, 2009, 200 (10) :1583-1592

[4] Streptococcus suis Meningitis, Hawaii [J].

Fittipaldi, Nahuel ;

Collis, Tarquin ;

Prothero, Bryscen ;

Gottschalk, Marcelo .

EMERGING INFECTIOUS DISEASES, 2009, 15 (12) :2067-2069

[5] Identification of proteins by matrix-assisted laser desorption ionization mass spectrometry following in-gel digestion in low-salt, nonvolatile buffer and simplified peptide recovery [J].

Fountoulakis, M ;

Langen, H .

ANALYTICAL BIOCHEMISTRY, 1997, 250 (02) :153-156

[6] Identification and characterization of novel immunogenic proteins of Streptococcus suis serotype 2 [J].

Geng, Hongran ;

Zhu, Li ;

Yuan, Yuan ;

Zhang, Wei ;

Li, Wenjun ;

Wang, Jie ;

Zheng, Yuling ;

Wei, Kaihua ;

Cao, Wuchun ;

Wang, Hengliang ;

Jiang, Yongqiang .

JOURNAL OF PROTEOME RESEARCH, 2008, 7 (09) :4132-4142

[7]

GILL DR, 1990, MOL MICROBIOL, V4, P575

[8] The pathogenesis of the meningitis caused by Streptococcus suis:: the unresolved questions [J].

Gottschalk, M ;

Segura, M .

VETERINARY MICROBIOLOGY, 2000, 76 (03) :259-272

[9]

Gottschalk Marcelo, 2007, Animal Health Research Reviews, V8, P29, DOI 10.1017/S1466252307001247

[10] DESCRIPTION OF 6 NEW CAPSULAR TYPES (29-34) OF STREPTOCOCCUS-SUIS [J].

HIGGINS, R ;

GOTTSCHALK, M ;

BOUDREAU, M ;

LEBRUN, A ;

HENRICHSEN, J .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1995, 7 (03) :405-406

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