A novel hydrophilic poly(lactide-co-glycolide)/lecithin hybrid microspheres sintered scaffold for bone repair

被引：34

作者：

Shi, Xuetao ^{[1
,2
]}

Wang, Yingjun ^{[1
,3
]}

Ren, Li ^{[1
,3
]}

Lai, Chen ^{[1
,3
]}

Gong, Yihong ^{[2
]}

Wang, Dong-An ^{[2
,3
]}

机构：

[1] S China Univ Technol, Sch Mat Sci & Engn, Guangzhou 510641, Peoples R China

[2] Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, Singapore 637457, Singapore

[3] S China Univ Technol, Key Lab Specially Funct Mat & Adv Mfg Technol, Minist Educ, Guangzhou 510641, Peoples R China

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2010年 / 92A卷 / 03期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

poly(lattice-co-glycolide); lecithin; scaffolds; bone repair; TISSUE; LECITHIN; DELIVERY; BIOCOMPATIBILITY; COMPOSITES; MATRICES; GROWTH; POLY(L-LACTIDE); MICROEMULSIONS; FABRICATION;

D O I：

10.1002/jbm.a.32423

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Novel 3-D Porous scaffolds made of sintered poly(lacide-co-glycolide) (PLGA)/lecithin hybrid microspheres (PLGA/Lec-SMS) were developed and investigated. The addition of lecithin in PLGA bulk Successfully managed the desired hydrophilic modification without sacrificing bulk properties. The outcomes were verified with infrared (ATR-FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and contact angle analyses. Specifically, this model of scaffold gained significant improvement in mechanical (mainly compressive) strength upon an optimization of lecithin fractions aligning with sintering conditions. Given a perspective of bone tissue engineering use, human fetal osteoblasts were seeded into a series of these PLGA/Lec-SMS scaffolds upon which key parameters of cytocompatibility and osteoconductivity (including cell viability, alkaline phosphatase activity, calcium secretion, and osteogenic genes expression) were assessed. Osteoblasts seeded on PLGA scaffolds with 5 wt % lecithin demonstrated high cell viability and alkaline phosphatase activity. Moreover, elevated lecithin also enhanced the expression of type I collagen. Taken together, these results suggest PLGA/Lec-SMS are promising scaffolds for bone repair. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 92A: 963-972, 2010

引用

页码：963 / 972

页数：10

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