Reactivity of ferrocenylcarbodiimide with DNA duplex containing single-mismatched base pairs

被引:3
作者
Mukumoto, Kosuke
Watanabe, Sadayoshi
Nojima, Takahiko
Waki, Michinori
Takenaka, Shigeori [1 ]
机构
[1] Kyushu Inst Technol, Dept Appl Chem, Fac Engn, Kitakyushu, Fukuoka 8048550, Japan
[2] Kyushu Univ, Dept Appl Chem, Fac Engn, Fukuoka 8190395, Japan
关键词
D O I
10.2116/analsci.23.645
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Ferrocenylcarbodiimide (1), which is known to react with a guanine (G) or thymine (T) base of single stranded DNA, was allowed to react with DNA duplex having a single mismatched base pair of G-T, T-T, or T-cytosine (C). Electrophoreograms of the reaction mixture showed that 1 could react with G or T base of the mismatched sites on the DNA duplex. However, I also reacted with the G base of the terminal site on the DNA duplex. This showed that 1 can react with an unpaired base or unstable base pair such as a terminal or mismatched base on the DNA duplex. Electrochemical mismatch detection could be achieved after hybridization of the ferrocenylated mismatched DNA duplex with a selected DNA probe-immobilized electrode. These results revealed that 1 has a potentiality of serving as a labeling reagent of mismatched bases on the DNA duplex, which is important in the search for heterozygous single nucleotide polymorphisms (SNPs).
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页码:645 / 649
页数:5
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