Knockdown Resistance (kdr) Mutations in Indian Anopheles stephensi (Diptera: Culicidae) Populations

被引：16
作者：
Dykes, Cherry L. [1 ]
Das, Manoj K. [2 ]
Eapen, Alex [3 ]
Batra, Chandra P. [1 ]
Ghosh, Susanta K. [4 ]
Vijayan, V. A. [5 ]
Mishra, Shobhna [1 ]
Singh, Om P. [1 ]
机构：
[1] Natl Inst Malaria Res, Sect 8, Dwarka 110077, India
[2] Natl Inst Malaria Res, Field Unit, TB Sanat Complex, Ranchi 835301, Bihar, India
[3] Natl Inst Malaria Res, Natl Inst Epidemiol Campus, Madras 600077, Tamil Nadu, India
[4] Natl Inst Malaria Res, Devanhalli 562110, Bengaluru, India
[5] Univ Mysore, Dept Studies Zool, Mysore 570006, Karnataka, India
来源：
JOURNAL OF MEDICAL ENTOMOLOGY | 2016年 / 53卷 / 02期
基金：
美国国家卫生研究院;
关键词：
Anopheles stephensi; voltage-gated sodium channel; knockdown resistance; insecticide resistance; GATED SODIUM-CHANNEL; MALARIA VECTOR; CULICIFACIES; PYRETHROIDS; DDT; PCR; SUSCEPTIBILITY; INSECTICIDES; SUBPICTUS; L1014S;
D O I：
10.1093/jme/tjv242
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
Knockdown resistance (kdr) in insects resulting from mutation(s) in the voltage-gated sodium channel (VGSC) gene is one of the mechanisms of resistance against DDT and the pyrethroid group of insecticides. Earlier, we reported the presence of two classic kdr mutations, i.e., L1014F and L1014S in Anopheles stephensi Liston, a major Indian malaria vector affecting mainly urban areas. This report presents the distribution of these alleles in different An. stephensi populations. Seven populations of An. stephensi from six states of India were screened for the presence of two alternative kdr mutations L1014F and L1014S using allele-specific polymerase chain reaction assays. We recorded the presence of both kdr mutations in northern Indian populations (Alwar and Gurgaon), with the preponderance of L1014S, whereas only L1014F was present in Raipur (central India) and Chennai (southern India). None of the kdr mutations were found in Ranchi in eastern India and in Mangaluru and Mysuru in southern India. This study provides evidence for a focal pattern of distribution of kdr alleles in India.
引用
收藏
页码：315 / 320
页数：6
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