Recent advances in nanopore-based nucleic acid analysis and sequencing

被引：12

作者：

Shi, Jidong ^{[1
]}

Hou, Junfeng ^{[1
,2
]}

Fang, Ying ^{[1
]}

机构：

[1] Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[2] Shandong Univ, Sch Ocean, Weihai 264209, Peoples R China

来源：

MICROCHIMICA ACTA | 2016年 / 183卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Nanopore; Nucleic acid; Nanotechnology; Gene sequencing; Nanomaterial; SOLID-STATE NANOPORE; SINGLE-STRANDED-DNA; POLYMERASE COMPLEXES; ALPHA-HEMOLYSIN; TRANS LOCATION; PROTEIN PORE; MOLECULES; TRANSLOCATION; GRAPHENE; DISCRIMINATION;

D O I：

10.1007/s00604-015-1503-y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nanopore-based sequencing platforms are transforming the field of genomic science. This review (containing 116 references) highlights some recent progress on nanopore-based nucleic acid analysis and sequencing. These studies are classified into three categories, biological, solid-state, and hybrid nanopores, according to their nanoporous materials. We begin with a brief description of the translocation-based detection mechanism of nanopores. Next, specific examples are given in nanopore-based nucleic acid analysis and sequencing, with an emphasis on identifying strategies that can improve the resolution of nanopores. This review concludes with a discussion of future research directions that will advance the practical applications of nanopore technology.

引用

页码：925 / 939

页数：15

共 116 条

[1] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules [J].